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Journal of Bacteriology, June 2006, p. 4111-4116, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.01949-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Periplasmic Folding of a Cysteineless Autotransporter Passenger Domain Interferes with Its Outer Membrane Translocation

Nancy Rutherford,1 Marie-Ève Charbonneau,1 Frédéric Berthiaume,1 Jean-Michel Betton,2 and Michael Mourez1*

Canada Research Chair on Bacterial Animal Diseases, Université de Montréal, Faculté de Médecine Vétérinaire, 3200 Sicotte, St.-Hyacinthe, J2S 7C6, Quebec, Canada,1 CNRS URA 2185, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France2

Received 20 December 2005/ Accepted 14 March 2006

Autotransporters are single polypeptides consisting of an outer membrane translocation domain mediating the translocation of a passenger domain. The periplasmic folding state of the passenger domain is controversial. By comparisons of passenger domains differing in their folding properties, our results suggest that periplasmic folding of passenger domains interferes with translocation.

* Corresponding author. Mailing address: Canada Research Chair on Bacterial Animal Diseases, Université de Montréal, Faculté de Médecine Vétérinaire, 3200 Sicotte, St.-Hyacinthe, J2S 7C6, Quebec, Canada. Phone: (450) 773-8521, ext. 8430. Fax: (450) 778-8108. E-mail: m.mourez{at}umontreal.ca.

Journal of Bacteriology, June 2006, p. 4111-4116, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.01949-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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