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Journal of Bacteriology, June 2006, p. 4153-4157, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.01926-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Xanthosine Utilization in Salmonella enterica Serovar Typhimurium Is Recovered by a Single Aspartate-to-Glycine Substitution in Xanthosine Phosphorylase

Michael Riis Hansen, Jesper Tranekjær Jørgensen, and Gert Dandanell*

Department of Biological Chemistry, Institute of Molecular Biology and Physiology, University of Copenhagen, Sølvgade 83 H, 1307 Copenhagen K, Denmark

Received 16 December 2005/ Accepted 20 March 2006

xapABR from Salmonella enterica was analyzed and compared with the corresponding Escherichia coli genes. xapB and xapR, but not xapA, encode functional proteins. An S. enterica XapA(Asp72Gly) mutant that restores the phosphorolytic activity was selected. The purified mutant enzyme has different kinetic constants than the E. coli enzyme but similar substrate specificity.

* Corresponding author. Mailing address: Department of Biological Chemistry, Institute of Molecular Biology and Physiology, University of Copenhagen, Sølvgade 83 H, 1307 Copenhagen K, Denmark. Phone: 45 35 32 20 25. Fax: 45 35 32 20 40. E-mail: dandanell{at}mermaid.molbio.ku.dk.

Journal of Bacteriology, June 2006, p. 4153-4157, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.01926-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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