This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kasiviswanathan, R. Articles by Kelman, Z. Search for Related Content PubMed PubMed Citation Articles by Kasiviswanathan, R. Articles by Kelman, Z.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2006, p. 4577-4580, Vol. 188, No. 12
0021-9193/06/$08.00+0     doi:10.1128/JB.00168-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

DNA Binding by the Methanothermobacter thermautotrophicus Cdc6 Protein Is Inhibited by the Minichromosome Maintenance Helicase

Rajesh Kasiviswanathan, Jae-Ho Shin, and Zvi Kelman^*

University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Drive, Rockville, Maryland 20850

Received 31 January 2006/ Accepted 5 April 2006

The Cdc6 proteins from the archaeon Methanothermobacter thermautotrophicus were previously shown to bind double-stranded DNA. It is shown here that the proteins also bind single-stranded DNA. Using minichromosome maintenance (MCM) helicase mutant proteins unable to bind DNA, it was found that the interaction of MCM with Cdc6 inhibits the DNA binding activity of Cdc6.

Present address: Department of Molecular Pharmacology, St. Jude Children's Research Hospital, 332 N. Lauderdale Street, Memphis, TN 38105.

This article has been cited by other articles:

• Haugland, G. T., Sakakibara, N., Pey, A. L., Rollor, C. R., Birkeland, N.-K., Kelman, Z. (2008). Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity. Nucleic Acids Res 36: 5602-5609 [Abstract] [Full Text]  
• Shin, J.-H., Heo, G. Y., Kelman, Z. (2008). The Methanothermobacter thermautotrophicus Cdc6-2 Protein, the Putative Helicase Loader, Dissociates the Minichromosome Maintenance Helicase. J. Bacteriol. 190: 4091-4094 [Abstract] [Full Text]  
• Sakakibara, N., Kasiviswanathan, R., Melamud, E., Han, M., Schwarz, F. P., Kelman, Z. (2008). Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein. Nucleic Acids Res 36: 1309-1320 [Abstract] [Full Text]  
• Gaudier, M., Schuwirth, B. S., Westcott, S. L., Wigley, D. B. (2007). Structural Basis of DNA Replication Origin Recognition by an ORC Protein. Science 317: 1213-1216 [Abstract] [Full Text]