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Journal of Bacteriology, July 2006, p. 4903-4917, Vol. 188, No. 13
0021-9193/06/$08.00+0     doi:10.1128/JB.00180-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

PopF1 and PopF2, Two Proteins Secreted by the Type III Protein Secretion System of Ralstonia solanacearum, Are Translocators Belonging to the HrpF/NopX Family{dagger}

Damien Meyer,1 Sébastien Cunnac,1,{ddagger} Mareva Guéneron,1,§ Céline Declercq,1,¶ Frédérique Van Gijsegem,2 Emmanuelle Lauber,1 Christian Boucher,1 and Matthieu Arlat1*

Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BP52627, 31326 Castanet tolosan Cedex,1 UMR INRA, INA-PG, Université Paris VI, Interactions Plantes Pathogènes, INA-PG, 16 rue Claude Bernard, 75231 Paris Cedex 05, France2

Received 3 February 2006/ Accepted 14 April 2006

Ralstonia solanacearum GMI1000 is a gram-negative plant pathogen which contains an hrp gene cluster which codes for a type III protein secretion system (TTSS). We identified two novel Hrp-secreted proteins, called PopF1 and PopF2, which display similarity to one another and to putative TTSS translocators, HrpF and NopX, from Xanthomonas spp. and rhizobia, respectively. They also show similarities with TTSS translocators of the YopB family from animal-pathogenic bacteria. Both popF1 and popF2 belong to the HrpB regulon and are required for the interaction with plants, but PopF1 seems to play a more important role in virulence and hypersensitive response (HR) elicitation than PopF2 under our experimental conditions. PopF1 and PopF2 are not necessary for the secretion of effector proteins, but they are required for the translocation of AvrA avirulence protein into tobacco cells. We conclude that PopF1 and PopF2 are type III translocators belonging to the HrpF/NopX family. The hrpF gene of Xanthomonas campestris pv. campestris partially restored HR-inducing ability to popF1 popF2 mutants of R. solanacearum, suggesting that translocators of R. solanacearum and Xanthomonas are functionally conserved. Finally, R. solanacearum strain UW551, which does not belong to the same phylotype as GMI1000, also possesses two putative translocator proteins. However, although one of these proteins is clearly related to PopF1 and PopF2, the other seems to be different and related to NopX proteins, thus showing that translocators might be variable in R. solanacearum.

* Corresponding author. Mailing address: Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BP52627, 31326 Castanet tolosan Cedex, France. Phone: (33) 561 28 50 47. Fax: (33) 561 28 50 47. E-mail: arlat{at}toulouse.inra.fr.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020.

§ Present address: Protisvalor, Jardin du Pharo, 13007 Marseille, France.

Present address: Plate-forme Transcriptome-Biopuces, Centre de Bioingenierie Gilbert Durand, Département de Génie Biochimique et Alimentaire, Institut National des Sciences Appliquées, 31077 Toulouse Cedex 4, France.

Journal of Bacteriology, July 2006, p. 4903-4917, Vol. 188, No. 13
0021-9193/06/$08.00+0     doi:10.1128/JB.00180-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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