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Journal of Bacteriology, August 2006, p. 5460-5468, Vol. 188, No. 15
0021-9193/06/$08.00+0     doi:10.1128/JB.00215-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Control of Acetyl-Coenzyme A Synthetase (AcsA) Activity by Acetylation/Deacetylation without NAD⁺ Involvement in Bacillus subtilis

Jeffrey G. Gardner,¹ Frank J. Grundy,² Tina M. Henkin,² and Jorge C. Escalante-Semerena^1*

Department of Bacteriology University of Wisconsin-Madison, Madison, Wisconsin,¹ Department of Microbiology, The Ohio State University, Columbus, Ohio²

Received 8 February 2006/ Accepted 12 May 2006

Posttranslational modification is an efficient mechanism for controlling the activity of structural proteins, gene expression regulators, and enzymes in response to rapidly changing physiological conditions. Here we report in vitro and in vivo evidence that the acuABC operon of the gram-positive soil bacterium Bacillus subtilis encodes a protein acetyltransferase (AcuA) and a protein deacetylase (AcuC), which may control the activity of acetyl-coenzyme A (CoA) synthetase (AMP-forming, AcsA) in this bacterium. Results from in vitro experiments using purified proteins show that AcsA is a substrate for the acetyl-CoA-dependent AcuA acetyltransferase. Mass spectrometry analysis of a tryptic digest of acetylated AcsA (AcsA^Ac) identified residue Lys549 as the sole modification site in the protein. Unlike sirtuins, the AcuC protein did not require NAD⁺ as cosubstrate to deacetylate AcsA^Ac. The function of the putative AcuB protein remains unknown.

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* Corresponding author: Department of Bacteriology, 144A Enzyme Institute, 1710 University Avenue, Madison, WI 53726-4087. Phone: (608) 262-7379. Fax: (608) 265-7909. E-mail: escalante{at}bact.wisc.edu.

Supplemental material for this article may be found at http://jb.asm.org/.

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Journal of Bacteriology, August 2006, p. 5460-5468, Vol. 188, No. 15
0021-9193/06/$08.00+0     doi:10.1128/JB.00215-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Gardner, J. G., Escalante-Semerena, J. C. (2009). In Bacillus subtilis, the Sirtuin Protein Deacetylase, Encoded by the srtN Gene (Formerly yhdZ), and Functions Encoded by the acuABC Genes Control the Activity of Acetyl Coenzyme A Synthetase. J. Bacteriol. 191: 1749-1755 [Abstract] [Full Text]  
• Gardner, J. G., Escalante-Semerena, J. C. (2008). Biochemical and Mutational Analyses of AcuA, the Acetyltransferase Enzyme That Controls the Activity of the Acetyl Coenzyme A Synthetase (AcsA) in Bacillus subtilis. J. Bacteriol. 190: 5132-5136 [Abstract] [Full Text]  
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