This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Choi, S.-Y.
Right arrow Articles by Zuber, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choi, S.-Y.
Right arrow Articles by Zuber, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2006, p. 5741-5751, Vol. 188, No. 16
0021-9193/06/$08.00+0     doi:10.1128/JB.00443-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Global Regulator Spx Functions in the Control of Organosulfur Metabolism in Bacillus subtilis{dagger}

Soon-Yong Choi,1,2 Dindo Reyes,1 Montira Leelakriangsak,1 and Peter Zuber1*

Department of Environmental & Biomolecular Systems, OGI School of Science & Engineering, Oregon Health & Science University, Beaverton, Oregon,1 Department of Microbiology, HanNam University, 133 Ojung-Dong, Taeduk-Ku, Daejon 306-791, South Korea2

Received 30 March 2006/ Accepted 2 June 2006

Spx is a global transcriptional regulator of the oxidative stress response in Bacillus subtilis. Its target is RNA polymerase, where it contacts the {alpha} subunit C-terminal domain. Recently, evidence was presented that Spx participates in sulfate-dependent control of organosulfur utilization operons, including the ytmI, yxeI, ssu, and yrrT operons. The yrrT operon includes the genes that function in cysteine synthesis from S-adenosylmethionine through intermediates S-adenosylhomocysteine, ribosylhomocysteine, homocysteine, and cystathionine. These operons are also negatively controlled by CymR, the repressor of cysteine biosynthesis operons. All of the operons are repressed in media containing cysteine or sulfate but are derepressed in medium containing the alternative sulfur source, methionine. Spx was found to negatively control the expression of these operons in sulfate medium, in part, by stimulating the expression of the cymR gene. In addition, microarray analysis, monitoring of yrrT-lacZ fusion expression, and in vitro transcription studies indicate that Spx directly activates yrrT operon expression during growth in medium containing methionine as sole sulfur source. These experiments have uncovered additional roles for Spx in the control of gene expression during unperturbed, steady-state growth.

* Corresponding author. Mailing address: EBS/OGI School of Science & Engineering, Oregon Health & Science University, Beaverton, OR 97006. Phone: (503) 748-7335. Fax: (503) 748-1464. E-mail: pzuber{at}ebs.ogi.edu.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, August 2006, p. 5741-5751, Vol. 188, No. 16
0021-9193/06/$08.00+0     doi:10.1128/JB.00443-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pother, D.-C., Liebeke, M., Hochgrafe, F., Antelmann, H., Becher, D., Lalk, M., Lindequist, U., Borovok, I., Cohen, G., Aharonowitz, Y., Hecker, M. (2009). Diamide Triggers Mainly S Thiolations in the Cytoplasmic Proteomes of Bacillus subtilis and Staphylococcus aureus. J. Bacteriol. 191: 7520-7530 [Abstract] [Full Text]  
  • Garg, S. K., Kommineni, S., Henslee, L., Zhang, Y., Zuber, P. (2009). The YjbH Protein of Bacillus subtilis Enhances ClpXP-Catalyzed Proteolysis of Spx. J. Bacteriol. 191: 1268-1277 [Abstract] [Full Text]  
  • Tanous, C., Soutourina, O., Raynal, B., Hullo, M.-F., Mervelet, P., Gilles, A.-M., Noirot, P., Danchin, A., England, P., Martin-Verstraete, I. (2008). The CymR Regulator in Complex with the Enzyme CysK Controls Cysteine Metabolism in Bacillus subtilis. J. Biol. Chem. 283: 35551-35560 [Abstract] [Full Text]  
  • Andre, G., Even, S., Putzer, H., Burguiere, P., Croux, C., Danchin, A., Martin-Verstraete, I., Soutourina, O. (2008). S-box and T-box riboswitches and antisense RNA control a sulfur metabolic operon of Clostridium acetobutylicum. Nucleic Acids Res 36: 5955-5969 [Abstract] [Full Text]  
  • Mitrophanov, A. Y., Groisman, E. A. (2008). Signal integration in bacterial two-component regulatory systems. Genes Dev. 22: 2601-2611 [Abstract] [Full Text]  
  • Hochgrafe, F., Mostertz, J., Pother, D.-C., Becher, D., Helmann, J. D., Hecker, M. (2007). S-Cysteinylation Is a General Mechanism for Thiol Protection of Bacillus subtilis Proteins after Oxidative Stress. J. Biol. Chem. 282: 25981-25985 [Abstract] [Full Text]  
  • Leelakriangsak, M., Kobayashi, K., Zuber, P. (2007). Dual Negative Control of spx Transcription Initiation from the P3 Promoter by Repressors PerR and YodB in Bacillus subtilis. J. Bacteriol. 189: 1736-1744 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»