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Two GacA-Dependent Small RNAs Modulate the Quorum-Sensing Response in Pseudomonas aeruginosa

Elisabeth Kay,^1, Bérénice Humair,¹ Valérie Dénervaud,¹ Kathrin Riedel,² Stéphanie Spahr,¹ Leo Eberl,² Claudio Valverde,^1, and Dieter Haas^1*

Département de Microbiologie Fondamentale, Université de Lausanne, CH-1015 Lausanne,¹ Institut für Pflanzenbiologie, Universität Zürich, CH-8008 Zürich, Switzerland²

Received 23 March 2006/ Accepted 26 May 2006

In Pseudomonas aeruginosa, the GacS/GacA two-component system positively controls the quorum-sensing machinery and the expression of extracellular products via two small regulatory RNAs, RsmY and RsmZ. An rsmY rsmZ double mutant and a gacA mutant were similarly impaired in the synthesis of the quorum-sensing signal N-butanoyl-homoserine lactone, the disulfide bond-forming enzyme DsbA, and the exoproducts hydrogen cyanide, pyocyanin, elastase, chitinase (ChiC), and chitin-binding protein (CbpD). Both mutants showed increased swarming ability, azurin release, and early biofilm development.

Supplemental material for this article may be found at http://jb.asm.org/.

Present address: Laboratoire Pathogénie et Adaptation des Micro-organismes, Institut Jean Roget, Université Joseph Fourier, F-38042 Grenoble, France.

Present address: Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal B1876BXD, Argentina.

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