This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rey, F. E.
Right arrow Articles by Harwood, C. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rey, F. E.
Right arrow Articles by Harwood, C. S.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2006, p. 6143-6152, Vol. 188, No. 17
0021-9193/06/$08.00+0     doi:10.1128/JB.00381-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Regulation of Uptake Hydrogenase and Effects of Hydrogen Utilization on Gene Expression in Rhodopseudomonas palustris

Federico E. Rey,1,2 Yasuhiro Oda,1 and Caroline S. Harwood1*

Department of Microbiology, University of Washington, Seattle, Washington 98195-7242,1 Department of Microbiology, University of Iowa, Iowa City, Iowa 522422

Received 16 March 2006/ Accepted 12 June 2006

Rhodopseudomonas palustris is a purple, facultatively phototrophic bacterium that uses hydrogen gas as an electron donor for carbon dioxide fixation during photoautotrophic growth or for ammonia synthesis during nitrogen fixation. It also uses hydrogen as an electron supplement to enable the complete assimilation of oxidized carbon compounds, such as malate, into cell material during photoheterotrophic growth. The R. palustris genome predicts a membrane-bound nickel-iron uptake hydrogenase and several regulatory proteins to control hydrogenase synthesis. There is also a novel sensor kinase gene (RPA0981) directly adjacent to the hydrogenase gene cluster. Here we show that the R. palustris regulatory sensor hydrogenase HupUV acts in conjunction with the sensor kinase-response regulator protein pair HoxJ-HoxA to activate hydrogenase expression in response to hydrogen gas. Transcriptome analysis indicated that the HupUV-HoxJA regulatory system also controls the expression of genes encoding a predicted dicarboxylic acid transport system, a putative formate transporter, and a glutamine synthetase. RPA0981 had a small effect in repressing hydrogenase synthesis. We also determined that the two-component system RegS-RegR repressed expression of the uptake hydrogenase, probably in response to changes in intracellular redox status. Transcriptome analysis indicated that about 30 genes were differentially expressed in R. palustris cells that utilized hydrogen when growing photoheterotrophically on malate under nitrogen-fixing conditions compared to a mutant strain that lacked uptake hydrogenase. From this it appears that the recycling of reductant in the form of hydrogen does not have extensive nonspecific effects on gene expression in R. palustris.

* Corresponding author. Mailing address: Department of Microbiology, Box 357242, 1959 N.E. Pacific Street, University of Washington, Seattle, WA 98195-7242. Phone: (206) 221-2848. Fax: (206) 221-5041. E-mail: csh5{at}u.washington.edu.

Journal of Bacteriology, September 2006, p. 6143-6152, Vol. 188, No. 17
0021-9193/06/$08.00+0     doi:10.1128/JB.00381-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sattley, W. M., Madigan, M. T., Swingley, W. D., Cheung, P. C., Clocksin, K. M., Conrad, A. L., Dejesa, L. C., Honchak, B. M., Jung, D. O., Karbach, L. E., Kurdoglu, A., Lahiri, S., Mastrian, S. D., Page, L. E., Taylor, H. L., Wang, Z. T., Raymond, J., Chen, M., Blankenship, R. E., Touchman, J. W. (2008). The Genome of Heliobacterium modesticaldum, a Phototrophic Representative of the Firmicutes Containing the Simplest Photosynthetic Apparatus. J. Bacteriol. 190: 4687-4696 [Abstract] [Full Text]  
  • Pan, C., Oda, Y., Lankford, P. K., Zhang, B., Samatova, N. F., Pelletier, D. A., Harwood, C. S., Hettich, R. L. (2008). Characterization of Anaerobic Catabolism of p-Coumarate in Rhodopseudomonas palustris by Integrating Transcriptomics and Quantitative Proteomics. Mol. Cell. Proteomics 7: 938-948 [Abstract] [Full Text]  
  • Lindemann, A., Moser, A., Pessi, G., Hauser, F., Friberg, M., Hennecke, H., Fischer, H.-M. (2007). New Target Genes Controlled by the Bradyrhizobium japonicum Two-Component Regulatory System RegSR. J. Bacteriol. 189: 8928-8943 [Abstract] [Full Text]  
  • Rey, F. E., Heiniger, E. K., Harwood, C. S. (2007). Redirection of Metabolism for Biological Hydrogen Production. Appl. Environ. Microbiol. 73: 1665-1671 [Abstract] [Full Text]  
  • Peres, C. M., Harwood, C. S. (2006). BadM Is a Transcriptional Repressor and One of Three Regulators That Control Benzoyl Coenzyme A Reductase Gene Expression in Rhodopseudomonas palustris. J. Bacteriol. 188: 8662-8665 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»