This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gulmezian, M. Articles by Clarke, C. F. Search for Related Content PubMed PubMed Citation Articles by Gulmezian, M. Articles by Clarke, C. F.

Genetic Evidence for an Interaction of the UbiG O-Methyltransferase with UbiX in Escherichia coli Coenzyme Q Biosynthesis

Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095-1569,¹ Department of Biochemistry, Loma Linda University School of Medicine, Loma Linda, California 92354²

Received 10 May 2006/ Accepted 22 June 2006

IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiX_K-12 reverses this phenotype, suggesting that UbiX may interact with UbiG.