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 Previous Article

Journal of Bacteriology, September 2006, p. 6443-6448, Vol. 188, No. 17
0021-9193/06/$08.00+0     doi:10.1128/JB.00266-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Superoxide Dismutase B Gene (sodB)-Deficient Mutants of Francisella tularensis Demonstrate Hypersensitivity to Oxidative Stress and Attenuated Virulence

Chandra Shekhar Bakshi,1* Meenakshi Malik,1 Kevin Regan,1 J. Andres Melendez,1 Dennis W. Metzger,1 Vitaly M. Pavlov,2 and Timothy J. Sellati1*

Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208,1 State Research Center for Applied Microbiology, Obolensk, Russia2

Received 20 February 2006/ Accepted 26 June 2006

A Francisella tularensis live vaccine strain mutant (sodBFt) with reduced Fe-superoxide dismutase gene expression was generated and found to exhibit decreased sodB activity and increased sensitivity to redox cycling compounds compared to wild-type bacteria. The sodBFt mutant also was significantly attenuated for virulence in mice. Thus, this study has identified sodB as an important F. tularensis virulence factor.

* Corresponding author. Mailing address: Center for Immunology and Microbial Disease, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208-3479. Phone for Timothy J. Sellati: (518) 262-8140. Fax: (518) 262-2885. E-mail: sellatt{at}mail.amc.edu. Phone for Chandra Shekhar Bakshi: (518) 262-6263. Fax: (518) 262-6161. E-mail: bakshis{at}mail.amc.edu.

Journal of Bacteriology, September 2006, p. 6443-6448, Vol. 188, No. 17
0021-9193/06/$08.00+0     doi:10.1128/JB.00266-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schulert, G. S., McCaffrey, R. L., Buchan, B. W., Lindemann, S. R., Hollenback, C., Jones, B. D., Allen, L.-A. H. (2009). Francisella tularensis Genes Required for Inhibition of the Neutrophil Respiratory Burst and Intramacrophage Growth Identified by Random Transposon Mutagenesis of Strain LVS. Infect. Immun. 77: 1324-1336 [Abstract] [Full Text]  
  • Cybulski, R. J. Jr., Sanz, P., Alem, F., Stibitz, S., Bull, R. L., O'Brien, A. D. (2009). Four Superoxide Dismutases Contribute to Bacillus anthracis Virulence and Provide Spores with Redundant Protection from Oxidative Stress. Infect. Immun. 77: 274-285 [Abstract] [Full Text]  
  • Lindgren, H., Shen, H., Zingmark, C., Golovliov, I., Conlan, W., Sjostedt, A. (2007). Resistance of Francisella tularensis Strains against Reactive Nitrogen and Oxygen Species with Special Reference to the Role of KatG. Infect. Immun. 75: 1303-1309 [Abstract] [Full Text]  
  • Malik, M., Bakshi, C. S., McCabe, K., Catlett, S. V., Shah, A., Singh, R., Jackson, P. L., Gaggar, A., Metzger, D. W., Melendez, J. A., Blalock, J. E., Sellati, T. J. (2007). Matrix Metalloproteinase 9 Activity Enhances Host Susceptibility to Pulmonary Infection with Type A and B Strains of Francisella tularensis. J. Immunol. 178: 1013-1020 [Abstract] [Full Text]  


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