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Journal of Bacteriology, September 2006, p. 6694-6699, Vol. 188, No. 18
0021-9193/06/$08.00+0     doi:10.1128/JB.00509-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Localized Induction of the ntcA Regulatory Gene in Developing Heterocysts of Anabaena sp. Strain PCC 7120

Elvira Olmedo-Verd, Alicia M. Muro-Pastor, Enrique Flores,* and Antonia Herrero

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, E-41092 Seville, Spain

Received 10 April 2006/ Accepted 19 June 2006

The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N2-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P2) and promoters that are induced upon N step-down (P1 and P3). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P3 and P1.

* Corresponding author. Mailing address: Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Américo Vespucio 49, E-41092 Seville, Spain. Phone: 34 954 489523. Fax: 34 954 460065. E-mail: eflores{at}ibvf.csic.es.

Journal of Bacteriology, September 2006, p. 6694-6699, Vol. 188, No. 18
0021-9193/06/$08.00+0     doi:10.1128/JB.00509-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Muro-Pastor, A. M., Flores, E., Herrero, A. (2009). NtcA-Regulated Heterocyst Differentiation Genes hetC and devB from Anabaena sp. Strain PCC 7120 Exhibit a Similar Tandem Promoter Arrangement. J. Bacteriol. 191: 5765-5774 [Abstract] [Full Text]  
  • Paz-Yepes, J., Flores, E., Herrero, A. (2009). Expression and Mutational Analysis of the glnB Genomic Region in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 191: 2353-2361 [Abstract] [Full Text]  
  • Olmedo-Verd, E., Valladares, A., Flores, E., Herrero, A., Muro-Pastor, A. M. (2008). Role of Two NtcA-Binding Sites in the Complex ntcA Gene Promoter of the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 190: 7584-7590 [Abstract] [Full Text]  
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  • Moslavac, S., Nicolaisen, K., Mirus, O., Al Dehni, F., Pernil, R., Flores, E., Maldener, I., Schleiff, E. (2007). A TolC-Like Protein Is Required for Heterocyst Development in Anabaena sp. Strain PCC 7120. J. Bacteriol. 189: 7887-7895 [Abstract] [Full Text]  
  • Flores, E., Pernil, R., Muro-Pastor, A. M., Mariscal, V., Maldener, I., Lechno-Yossef, S., Fan, Q., Wolk, C. P., Herrero, A. (2007). Septum-Localized Protein Required for Filament Integrity and Diazotrophy in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 189: 3884-3890 [Abstract] [Full Text]  


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