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Journal of Bacteriology, September 2006, p. 6703-6708, Vol. 188, No. 18
0021-9193/06/$08.00+0     doi:10.1128/JB.00799-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

FliT Acts as an Anti-FlhD2C2 Factor in the Transcriptional Control of the Flagellar Regulon in Salmonella enterica Serovar Typhimurium

Shouji Yamamoto{dagger} and Kazuhiro Kutsukake*

Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University, Tsushima-Naka 3-1-1, Okayama 700-8530, Japan

Received 5 June 2006/ Accepted 7 July 2006

Flagellar operons are divided into three classes with respect to their transcriptional hierarchy in Salmonella enterica serovar Typhimurium. The class 1 gene products FlhD and FlhC act together in an FlhD2C2 heterotetramer, which binds upstream of the class 2 promoters to facilitate binding of RNA polymerase. Class 2 expression is known to be enhanced by a disruption mutation in a flagellar gene, fliT. In this study, we purified FliT protein in a His-tagged form and showed that the protein prevented binding of FlhD2C2 to the class 2 promoter and inhibited FlhD2C2-dependent transcription. Pull-down and far-Western blotting analyses revealed that the FliT protein was capable of binding to FlhD2C2 and FlhC and not to FlhD alone. We conclude that FliT acts as an anti-FlhD2C2 factor, which binds to FlhD2C2 through interaction with the FlhC subunit and inhibits its binding to the class 2 promoter.

* Corresponding author. Mailing address: Department of Biology, Faculty of Science, Okayama University, Tsushima-Naka 3-1-1, Okayama 700-8530, Japan. Phone: 81-86-251-7863. Fax: 81-86-251-7876. E-mail: ktkk{at}cc.okayama-u.ac.jp.

{dagger} Present address: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.

Journal of Bacteriology, September 2006, p. 6703-6708, Vol. 188, No. 18
0021-9193/06/$08.00+0     doi:10.1128/JB.00799-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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