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Journal of Bacteriology, January 2006, p. 464-468, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.464-468.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

High-Affinity Vanadate Transport System in the Cyanobacterium Anabaena variabilis ATCC 29413

Brenda S. Pratte and Teresa Thiel*

Department of Biology, University of Missouri—St. Louis, St. Louis, Missouri 63121-4499

Received 19 August 2005/ Accepted 21 October 2005

High-affinity vanadate transport systems have not heretofore been identified in any organism. Anabaena variabilis, which can fix nitrogen by using an alternative V-dependent nitrogenase, transported vanadate well. The concentration of vanadate giving half-maximum V-nitrogenase activity when added to V-starved cells was about 3 x 10–9 M. The genes for an ABC-type vanadate transport system, vupABC, were found in A. variabilis about 5 kb from the major cluster of genes encoding the V-nitrogenase, and like those genes, the vupABC genes were repressed by molybdate; however, unlike the V-nitrogenase genes the vanadate transport genes were expressed in vegetative cells. A vupB mutant failed to grow by using V-nitrogenase unless high levels of vanadate were provided, suggesting that there was also a low-affinity vanadate transport system that functioned in the vupB mutant. The vupABC genes belong to a family of putative metal transport genes that include only one other characterized transport system, the tungstate transport genes of Eubacterium acidaminophilum. Similar genes are not present in the complete genomes of other bacterial strains that have a V-nitrogenase, including Azotobacter vinelandii, Rhodopseudomonas palustris, and Methanosarcina barkeri.

* Corresponding author. Mailing address: Department of Biology, University of Missouri—St. Louis, One University Blvd., St. Louis, MO 63121-4499. Phone: (314) 516-6208. Fax: (314) 516-6233. E-mail: thiel{at}umsl.edu.

Journal of Bacteriology, January 2006, p. 464-468, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.464-468.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ungerer, J. L., Pratte, B. S., Thiel, T. (2008). Regulation of Fructose Transport and Its Effect on Fructose Toxicity in Anabaena spp.. J. Bacteriol. 190: 8115-8125 [Abstract] [Full Text]  
  • Weyman, P. D., Pratte, B., Thiel, T. (2008). Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen. Appl. Environ. Microbiol. 74: 2103-2110 [Abstract] [Full Text]  
  • Bellenger, J. P., Wichard, T., Kraepiel, A. M. L. (2008). Vanadium Requirements and Uptake Kinetics in the Dinitrogen-Fixing Bacterium Azotobacter vinelandii. Appl. Environ. Microbiol. 74: 1478-1484 [Abstract] [Full Text]  
  • Bevers, L. E., Hagedoorn, P.-L., Krijger, G. C., Hagen, W. R. (2006). Tungsten Transport Protein A (WtpA) in Pyrococcus furiosus: the First Member of a New Class of Tungstate and Molybdate Transporters.. J. Bacteriol. 188: 6498-6505 [Abstract] [Full Text]  
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