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Journal of Bacteriology, January 2006, p. 569-575, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.569-575.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Synthesis, Stability, and Function of Cytadhesin P1 and Accessory Protein B/C Complex of Mycoplasma pneumoniae

Robert H. Waldo III{dagger} and Duncan C. Krause*

Department of Microbiology, University of Georgia, Athens, Georgia

Received 4 August 2005/ Accepted 19 October 2005

The genes MPN141 and MPN142 encode the major adhesin P1 and the cytadherence-related B/C proteins (P90/P40), respectively, in Mycoplasma pneumoniae. Using reverse transcriptase PCR we found open reading frames MPN140 to MPN142 constitute a polycistronic transcriptional unit. Cytadherence mutant IV-22 has a frameshift mutation in MPN141 and lacks the P1, B, or C proteins. Recombinant MPN141 and/or MPN142 were introduced into mutant IV-22 by transposon delivery in several configurations, and the levels of the P1, B, and C proteins were assessed by immunoblotting. MPN142 in mutant IV-22 has a wild-type nucleotide sequence, yet the introduction of recombinant MPN141 alone to mutant IV-22, although it restored P1 levels, failed to restore levels of B or C. In contrast, recombinant MPN141 and MPN142 delivered in cis or in trans were sufficient to restore all three proteins. Taken together, our data indicated that some but not all synthesis of B or C is dependent on coupling to the translation of P1 immediately upstream of MPN142 and demonstrated that proteins B and C are not stable in the absence of P1. The linkage of MPN141 and MPN142 at the levels of transcription, translation, and protein stability, in addition to their previously demonstrated colocalization and the requirement of B and/or C for P1 function, reinforces the conclusion that these proteins constitute a multiprotein complex that functions in receptor binding.

* Corresponding author. Mailing address: Department of Microbiology, 523 Biological Sciences Building, University of Georgia, Athens, GA 30602. Phone: (706) 542-2671. Fax: (706) 542-2674. E-mail: dkrause{at}uga.edu.

{dagger} Present address: University of Tennessee Health Science Center, Memphis, TN 38163.

Journal of Bacteriology, January 2006, p. 569-575, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.569-575.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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