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Journal of Bacteriology, January 2006, p. 801-804, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.801-804.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Analysis of hemF Gene Function and Expression in Rhodobacter sphaeroides 2.4.1

Jill H. Zeilstra-Ryalls* and Kathryn L. Schornberg{dagger}

Department of Biological Sciences, Oakland University, Rochester, Michigan 48309

Received 11 September 2005/ Accepted 1 November 2005

The hemF gene of Rhodobacter sphaeroides 2.4.1 is predicted to code for an oxygen-dependent coproporphyrinogen III oxidase. We found that a HemF– mutant strain is unable to grow under aerobic conditions. We also determined that hemF expression is controlled by oxygen, which is mediated, at least in part, by the response regulatory protein PrrA.

* Corresponding author. Mailing address: Department of Biological Sciences, 374 Dodge Hall, Oakland University, Rochester, MI 48309. Phone: (248) 370-4497. Fax: (248) 370-4225. E-mail: zeilstra{at}oakland.edu.

{dagger} Present address: Department of Microbiology, University of Virginia, Charlottesville, VA 22908.

Journal of Bacteriology, January 2006, p. 801-804, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.801-804.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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