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Journal of Bacteriology, January 2006, p. 815-819, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.815-819.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Quorum-Sensing Negative Regulator RsaL of Pseudomonas aeruginosa Binds to the lasI Promoter

Giordano Rampioni,1 Iris Bertani,2 Elisabetta Zennaro,1 Fabio Polticelli,1 Vittorio Venturi,2 and Livia Leoni1*

Department of Biology, University "Roma Tre," Viale G. Marconi 446, 00146, Rome, Italy,1 Bacteriology Group, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy2

Received 25 August 2005/ Accepted 28 October 2005

A mutation in the rsaL gene of Pseudomonas aeruginosa produces dramatically higher amounts of N-acyl homoserine lactone with respect to the wild type, highlighting the key role of this negative regulator in controlling quorum sensing (QS) in this opportunistic pathogen. The DNA binding site of the RsaL protein on the rsaL-lasI bidirectional promoter partially overlaps the binding site of the LasR protein, consistent with the hypothesis that RsaL and LasR could be in binding competition on this promoter. This is the first direct demonstration that RsaL acts as a QS negative regulator by binding to the lasI promoter.

* Corresponding author. Mailing address: Department of Biology, University "Roma Tre," Viale G. Marconi 446, 00146, Rome, Italy. Phone: 39 0655176351. Fax: 39 0655176321. E-mail: leoni{at}bio.uniroma3.it.

Journal of Bacteriology, January 2006, p. 815-819, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.815-819.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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