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The ^E Cell Envelope Stress Response of Streptomyces coelicolor Is Influenced by a Novel Lipoprotein, CseA

Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom,¹ Biomolecular and Biomedical Research Centre, School of Applied Science, Northumbria University, Newcastle upon Tyne NE1 8ST, United Kingdom²

Received 8 June 2006/ Accepted 1 August 2006

We have investigated the role of CseA in the ^E cell envelope stress response of the gram-positive bacterium Streptomyces coelicolor. ^E is an extracytoplasmic function RNA polymerase sigma factor required for normal cell envelope integrity in S. coelicolor. ^E is encoded within a four-gene operon that also encodes CseA, a protein of unknown function, CseB, a response regulator and CseC, a transmembrane sensor histidine kinase (Cse represents control of sigma E). Previous work has shown that transcription of the sigE gene is completely dependent on the CseBC two-component system and that the CseBC-^E signal transduction system is induced by a wide variety of cell-wall-damaging agents. Here we address the role of CseA, a protein with no homologues outside the streptomycetes. We show that CseA is a novel lipoprotein localized to the extracytoplasmic face of the cell membrane and that loss of CseA results in upregulation of the sigE promoter.

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