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Replisome Localization in Vegetative and Aerial Hyphae of Streptomyces coelicolor

Beata Ruban-Omiaowska,^1,2, Dagmara Jakimowicz,^1,2, Aleksandra Smulczyk-Krawczyszyn,¹ Keith F. Chater,² and Jolanta Zakrzewska-Czerwiska^1*

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114 Wrocaw, Poland,¹ John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom²

Received 29 June 2006/ Accepted 27 July 2006

Using a functional fusion of DnaN to enhanced green fluorescent protein, we examined the subcellular localization of the replisome machinery in the vegetative mycelium and aerial mycelium of the multinucleoid organism Streptomyces coelicolor. Chromosome replication took place in many compartments of both types of hypha, with the apical compartments of the aerial mycelium exhibiting the highest replication activity. Within a single compartment, the number of "current" ongoing DNA replications was lower than the expected chromosome number, and the appearance of fluorescent foci was often heterogeneous, indicating that this process is asynchronous within compartments and that only selected chromosomes undergo replication.

B.R.-O. and D.J. contributed equally to this work.

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