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Journal of Bacteriology, November 2006, p. 7396-7404, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.01031-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Multiple Interactions between the Transmembrane Division Proteins of Bacillus subtilis and the Role of FtsL Instability in Divisome Assembly{triangledown}

Richard A. Daniel,1* Marie-Françoise Noirot-Gros,2 Philippe Noirot,2 and Jeff Errington1

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom,1 Laboratoire de Génétique Microbienne, Domaine de Vilvert, INRA, 78352 Jouy en Josas Cedex, France2

Received 13 July 2006/ Accepted 5 August 2006

About 11 essential proteins assemble into a ring structure at the surface of the cell to bring about cytokinesis in bacteria. Several of these proteins have their major domains located outside the membrane, forming an assembly that we call the outer ring (OR). Previous work on division in Bacillus subtilis has shown that four of the OR proteins—FtsL, DivIC, DivIB, and PBP 2B—are interdependent for assembly. This contrasts with the mainly linear pathway for the equivalent proteins in Escherichia coli. Here we show that the interdependent nature of the B. subtilis pathway could be due to effects on FtsL and DivIC stability and that DivIB is an important player in regulating this turnover. Two-hybrid approaches suggest that a multiplicity of protein-protein interactions contribute to the assembly of the OR. DivIC is unusual in interacting strongly only with FtsL. We propose a model for the formation of the OR through the mutual association of the membrane proteins directed by the cytosolic inner-ring proteins.

* Corresponding author. Mailing address: Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Phone: 0191 2228866. Fax: 0191 2227424. E-mail: Richard.Daniel{at}ncl.ac.uk.

{triangledown} Published ahead of print on 25 August 2006.

Journal of Bacteriology, November 2006, p. 7396-7404, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.01031-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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