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Journal of Bacteriology, November 2006, p. 7609-7616, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.01116-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Localization of the Transglutaminase Cross-Linking Sites in the Bacillus subtilis Spore Coat Protein GerQ{triangledown}

Alicia Monroe and Peter Setlow*

Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030

Received 26 July 2006/ Accepted 16 August 2006

The Bacillus subtilis spore coat protein GerQ is necessary for the proper localization of CwlJ, an enzyme important in the hydrolysis of the peptidoglycan cortex during spore germination. GerQ is cross-linked into high-molecular-mass complexes in the spore coat late in sporulation, and this cross-linking is largely due to a transglutaminase. This enzyme forms an {varepsilon}-({gamma}-glutamyl) lysine isopeptide bond between a lysine donor from one protein and a glutamine acceptor from another protein. In the current work, we have identified the residues in GerQ that are essential for transglutaminase-mediated cross-linking. We show that GerQ is a lysine donor and that any one of three lysine residues near the amino terminus of the protein (K2, K4, or K5) is necessary to form cross-links with binding partners in the spore coat. This leads to the conclusion that all Tgl-dependent GerQ cross-linking takes place via these three lysine residues. However, while the presence of any of these three lysine residues is essential for GerQ cross-linking, they are not essential for the function of GerQ in CwlJ localization.

* Corresponding author. Mailing address: Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030. Phone: (860) 679-2607. Fax: (860) 679-3408. E-mail: setlow{at}nso2.uchc.edu.

{triangledown} Published ahead of print on 25 August 2006.

Journal of Bacteriology, November 2006, p. 7609-7616, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.01116-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Carroll, A. M., Plomp, M., Malkin, A. J., Setlow, P. (2008). Protozoal Digestion of Coat-Defective Bacillus subtilis Spores Produces "Rinds" Composed of Insoluble Coat Protein. Appl. Environ. Microbiol. 74: 5875-5881 [Abstract] [Full Text]  


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