This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Domínguez-Ferreras, A.
Right arrow Articles by Sanjuán, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Domínguez-Ferreras, A.
Right arrow Articles by Sanjuán, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2006, p. 7617-7625, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.00719-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti{triangledown} ,{dagger}

Ana Domínguez-Ferreras,1 Rebeca Pérez-Arnedo,1 Anke Becker,2 José Olivares,1 María J. Soto,1 and Juan Sanjuán1*

Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Granada, Spain,1 Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, D-33501 Bielefeld, Germany2

Received 19 May 2006/ Accepted 7 August 2006

In this work, DNA microarrays were used to investigate genome-wide transcriptional responses of Sinorhizobium meliloti to a sudden increase in external osmolarity elicited by addition of either NaCl or sucrose to exponentially growing cultures. A time course of the response within the first 4 h after the osmotic shock was established. We found that there was a general redundancy in the differentially expressed genes after NaCl or sucrose addition. Both kinds of stress resulted in induction of a large number of genes having unknown functions and in repression of many genes coding for proteins with known functions. There was a strong replicon bias in the pattern of the osmotic stress response; whereas 64% of the upregulated genes had a plasmid localization, 85% of the downregulated genes were chromosomal. Among the pSymB osmoresponsive genes, 83% were upregulated, suggesting the importance of this plasmid for S. meliloti osmoadaptation. Indeed, we identified a 200-kb region in pSymB needed for adaptation to saline shock which has a high density of osmoregulated genes.

* Corresponding author. Mailing address: Estación Experimental del Zaidin, CSIC, Prof. Albareda 1, E-18008 Granada, Spain. Phone: 34-958-181600, ext. 259. Fax: 34-958-129600. E-mail: Juan.Sanjuan{at}eez.csic.es.

{triangledown} Published ahead of print on 17 August 2006.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2006, p. 7617-7625, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.00719-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Martinez-Salazar, J. M., Salazar, E., Encarnacion, S., Ramirez-Romero, M. A., Rivera, J. (2009). Role of the Extracytoplasmic Function Sigma Factor RpoE4 in Oxidative and Osmotic Stress Responses in Rhizobium etli. J. Bacteriol. 191: 4122-4132 [Abstract] [Full Text]  
  • Karunakaran, R., Ramachandran, V. K., Seaman, J. C., East, A. K., Mouhsine, B., Mauchline, T. H., Prell, J., Skeffington, A., Poole, P. S. (2009). Transcriptomic Analysis of Rhizobium leguminosarum Biovar viciae in Symbiosis with Host Plants Pisum sativum and Vicia cracca. J. Bacteriol. 191: 4002-4014 [Abstract] [Full Text]  
  • Bianco, C., Defez, R. (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain. J Exp Bot 0: erp140v1-erp140 [Abstract] [Full Text]  
  • Dominguez-Ferreras, A., Munoz, S., Olivares, J., Soto, M. J., Sanjuan, J. (2009). Role of Potassium Uptake Systems in Sinorhizobium meliloti Osmoadaptation and Symbiotic Performance. J. Bacteriol. 191: 2133-2143 [Abstract] [Full Text]  
  • Martinez-Salazar, J. M., Sandoval-Calderon, M., Guo, X., Castillo-Ramirez, S., Reyes, A., Loza, M. G., Rivera, J., Alvarado-Affantranger, X., Sanchez, F., Gonzalez, V., Davila, G., Ramirez-Romero, M. A. (2009). The Rhizobium etli RpoH1 and RpoH2 sigma factors are involved in different stress responses. Microbiology 155: 386-397 [Abstract] [Full Text]  
  • Ramos-Vega, A. L., Davila-Martinez, Y., Sohlenkamp, C., Contreras-Martinez, S., Encarnacion, S., Geiger, O., Lopez-Lara, I. M. (2009). SMb20651 is another acyl carrier protein from Sinorhizobium meliloti. Microbiology 155: 257-267 [Abstract] [Full Text]  
  • Rossbach, S., Mai, D. J., Carter, E. L., Sauviac, L., Capela, D., Bruand, C., de Bruijn, F. J. (2008). Response of Sinorhizobium meliloti to Elevated Concentrations of Cadmium and Zinc. Appl. Environ. Microbiol. 74: 4218-4221 [Abstract] [Full Text]  
  • Gourion, B., Francez-Charlot, A., Vorholt, J. A. (2008). PhyR Is Involved in the General Stress Response of Methylobacterium extorquens AM1. J. Bacteriol. 190: 1027-1035 [Abstract] [Full Text]  
  • McIntyre, H. J., Davies, H., Hore, T. A., Miller, S. H., Dufour, J.-P., Ronson, C. W. (2007). Trehalose Biosynthesis in Rhizobium leguminosarum bv. trifolii and Its Role in Desiccation Tolerance. Appl. Environ. Microbiol. 73: 3984-3992 [Abstract] [Full Text]  
  • Sauviac, L., Philippe, H., Phok, K., Bruand, C. (2007). An Extracytoplasmic Function Sigma Factor Acts as a General Stress Response Regulator in Sinorhizobium meliloti. J. Bacteriol. 189: 4204-4216 [Abstract] [Full Text]  
  • Vriezen, J. A. C., de Bruijn, F. J., Nusslein, K. (2007). Responses of Rhizobia to Desiccation in Relation to Osmotic Stress, Oxygen, and Temperature. Appl. Environ. Microbiol. 73: 3451-3459 [Full Text]  
  • MacLean, A. M., Finan, T. M., Sadowsky, M. J. (2007). Genomes of the Symbiotic Nitrogen-Fixing Bacteria of Legumes. Plant Physiol. 144: 615-622 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»