JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JB.00942-06v1
188/22/7876    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by De Gregorio, E.
Right arrow Articles by Di Nocera, P. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by De Gregorio, E.
Right arrow Articles by Di Nocera, P. P.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2006, p. 7876-7884, Vol. 188, No. 22
0021-9193/06/$08.00+0     doi:10.1128/JB.00942-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Structural Organization and Functional Properties of Miniature DNA Insertion Sequences in Yersiniae{triangledown}

Eliana De Gregorio, Giustina Silvestro, Rossella Venditti, Maria Stella Carlomagno, and Pier Paolo Di Nocera*

Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina, Università Federico II, Via S. Pansini 5, 80131 Napoli, Italy

Received 29 June 2006/ Accepted 14 August 2006

YPALs (Yersinia palindromic sequences) are miniature DNA insertions scattered along the chromosomes of yersiniae. The spread of these intergenic repeats likely occurred via transposition, as suggested by the presence of target site duplications at their termini and the identification of syntenic chromosomal regions which differ in the presence/absence of YPAL DNA among Yersinia strains. YPALs tend to be inserted closely downstream from the stop codon of flanking genes, and many YPAL targets overlap rho-independent transcriptional terminator-like sequences. This peculiar pattern of insertion supports the hypothesis that most of these repeats are cotranscribed with upstream sequences into mRNAs. YPAL RNAs fold into stable hairpins which may modulate mRNA decay. Accordingly, we found that YPAL-positive transcripts accumulate in Yersinia enterocolitica cells at significantly higher levels than homologous transcripts lacking YPAL sequences in their 3' untranslated region.

* Corresponding author. Mailing address: Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina, Università Federico II, Via S. Pansini 5, 80131 Naples, Italy. Phone: 0039-81-7462059. E-mail: dinocera{at}unina.it.

{triangledown} Published ahead of print on 8 September 2006.

Journal of Bacteriology, November 2006, p. 7876-7884, Vol. 188, No. 22
0021-9193/06/$08.00+0     doi:10.1128/JB.00942-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 2006 by the American Society for Microbiology. All rights reserved.