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Journal of Bacteriology, November 2006, p. 7932-7940, Vol. 188, No. 22
0021-9193/06/$08.00+0     doi:10.1128/JB.00964-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Two Chemosensory Operons of Rhodobacter sphaeroides Are Regulated Independently by Sigma 28 and Sigma 54

Angela C. Martin, Marcus Gould, Elaine Byles, Mark A. J. Roberts, and Judith P. Armitage^*

Microbiology Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom

Received 3 July 2006/ Accepted 28 August 2006

Rhodobacter sphaeroides has a complex chemosensory system, with several loci encoding multiple homologues of the components required for chemosensing in Escherichia coli. The operons cheOp2 and cheOp3 each encode complete pathways, and both are essential for chemosensing. The components of cheOp2 are predominantly localized to the cell pole, whereas those encoded by cheOp3 are predominantly targeted to a discrete cluster in the cytoplasm. Here we show that the expression of the two pathways is regulated independently. Overlapping promoters recognized by ²⁸ and ⁷⁰ RNAP holoenzyme transcribe cheOp2, whereas cheOp3 is regulated by one of the four ⁵⁴ homologues, RpoN3. The different regulation of these operons may reflect the need for balancing responses to extra- and intracellular signals under different growth conditions.

Published ahead of print on 8 September 2006.

A.C.M. and M.G. contributed equally to this study.

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