This Article Full Text Full Text (PDF) Other Versions of this Article: JB.01011-06v1 188/23/8145    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maresso, A. W. Articles by Schneewind, O. Search for Related Content PubMed PubMed Citation Articles by Maresso, A. W. Articles by Schneewind, O.

Surface Protein IsdC and Sortase B Are Required for Heme-Iron Scavenging of Bacillus anthracis

Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637

Received 11 July 2006/ Accepted 15 September 2006

Bacillus anthracis, the spore-forming agent of anthrax, requires iron for growth and is capable of scavenging heme-iron during infection. We show here that the B. anthracis iron-regulated surface determinants (isd) locus encompasses isdC, specifying a heme-iron binding surface protein. Anchoring of IsdC to the cell wall envelopes of vegetative bacilli requires srtB, which encodes sortase B. Purified sortase B cleaves IsdC between the threonine and the glycine of its NPKTG motif sorting signal. B. anthracis variants lacking either isdC or srtB display defects in heme-iron scavenging, suggesting that IsdC binding to heme-iron in the cell wall envelope contributes to bacterial uptake of heme.

Published ahead of print on 29 September 2006.

This article has been cited by other articles:

• Zhu, H., Xie, G., Liu, M., Olson, J. S., Fabian, M., Dooley, D. M., Lei, B. (2008). Pathway for Heme Uptake from Human Methemoglobin by the Iron-regulated Surface Determinants System of Staphylococcus aureus. J. Biol. Chem. 283: 18450-18460 [Abstract] [Full Text]  
• Liu, M., Tanaka, W. N., Zhu, H., Xie, G., Dooley, D. M., Lei, B. (2008). Direct Hemin Transfer from IsdA to IsdC in the Iron-regulated Surface Determinant (Isd) Heme Acquisition System of Staphylococcus aureus. J. Biol. Chem. 283: 6668-6676 [Abstract] [Full Text]  
• Maresso, A. W., Schneewind, O. (2008). Sortase as a Target of Anti-Infective Therapy. Pharmacol. Rev. 60: 128-141 [Abstract] [Full Text]  
• Marraffini, L. A., Schneewind, O. (2007). Sortase C-Mediated Anchoring of BasI to the Cell Wall Envelope of Bacillus anthracis. J. Bacteriol. 189: 6425-6436 [Abstract] [Full Text]