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Journal of Bacteriology, December 2006, p. 8153-8159, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.00966-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Detection of Protein-Protein Interactions in the Alkanesulfonate Monooxygenase System from Escherichia coli{triangledown}

Kholis Abdurachim and Holly R. Ellis*

Department of Chemistry and Biochemistry, Auburn University, Auburn, Alabama 36849

Received 3 July 2006/ Accepted 13 September 2006

The two-component alkanesulfonate monooxygenase system utilizes reduced flavin as a substrate to catalyze a unique desulfonation reaction during times of sulfur starvation. The importance of protein-protein interactions in the mechanism of flavin transfer was analyzed in these studies. The results from affinity chromatography and cross-linking experiments support the formation of a stable complex between the flavin mononucleotide (FMN) reductase (SsuE) and monooxygenase (SsuD). Interactions between the two proteins do not lead to overall conformational changes in protein structure, as indicated by the results from circular dichroism spectroscopy in the far-UV region. However, subtle changes in the flavin environment of FMN-bound SsuE that occur in the presence of SsuD were identified by circular dichroism spectroscopy in the visible region. These data are supported by the results from fluorescent spectroscopy experiments, where a dissociation constant of 0.0022 ± 0.0010 µM was obtained for the binding of SsuE to SsuD. Based on these studies, the stoichiometry for protein-protein interactions is proposed to involve a 1:1 monomeric association of SsuE with SsuD.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry, Auburn University, 179 Chemistry Building, Auburn, AL 36849. Phone: (334) 844-6991. Fax: (334) 844-6959. E-mail: ellishr{at}auburn.edu.

{triangledown} Published ahead of print on 22 September 2006.

Journal of Bacteriology, December 2006, p. 8153-8159, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.00966-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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