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Journal of Bacteriology, December 2006, p. 8321-8326, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.00865-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Revised Model for Enterococcus faecalis fsr Quorum-Sensing System: the Small Open Reading Frame fsrD Encodes the Gelatinase Biosynthesis-Activating Pheromone Propeptide Corresponding to Staphylococcal AgrD{triangledown} ,{dagger}

Jiro Nakayama,1* Shengmin Chen,1 Nozomi Oyama,1 Kenzo Nishiguchi,1 Essam A. Azab,1 Emi Tanaka,1 Reiko Kariyama,2 and Kenji Sonomoto1,3

Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School,1 Department of Functional Metabolic Design, Bio-architecture Center, Kyushu University, Fukuoka 812-8581, Japan,3 Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan2

Received 16 June 2006/ Accepted 8 September 2006

Gelatinase biosynthesis-activating pheromone (GBAP) is an autoinducing peptide involved in Enterococcus faecalis fsr quorum sensing, and its 11-amino-acid sequence has been identified in the C-terminal region of the 242-residue deduced fsrB product (J. Nakayama et al., Mol. Microbiol. 41:145-154, 2001). In this study, however, we demonstrated the existence of fsrD, encoding the GBAP propeptide, which is in frame with fsrB but is translated independently of fsrB. It was also demonstrated that FsrB', an FsrD segment-truncated FsrB, functions as a cysteine protease-like processing enzyme to generate GBAP from FsrD. This revised model is consistent with the staphylococcal agr system.

* Corresponding author. Mailing address: Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Phone: 81-92-642-3020. Fax: 81-92-642-3021. E-mail: nakayama{at}agr.kyushu-u.ac.jp.

{triangledown} Published ahead of print on 15 September 2006.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, December 2006, p. 8321-8326, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.00865-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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