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Journal of Bacteriology, December 2006, p. 8407-8412, Vol. 188, No. 24
0021-9193/06/$08.00+0     doi:10.1128/JB.00880-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

4-Chlorobenzoate Uptake in Comamonas sp. Strain DJ-12 Is Mediated by a Tripartite ATP-Independent Periplasmic Transporter{triangledown}

Jong-Chan Chae and Gerben J. Zylstra*

Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, New Jersey 08901

Received 19 June 2006/ Accepted 3 October 2006

The fcb gene cluster involved in the hydrolytic dehalogenation of 4-chlorobenzoate is organized in the order fcbB-fcbA-fcbT1-fcbT2-fcbT3-fcbC in Comamonas sp. strain DJ-12. The genes are operonic and inducible with 4-chloro-, 4-iodo-, and 4-bromobenzoate. The fcbT1, fcbT2, and fcbT3 genes encode a transporter in the secondary TRAP (tripartite ATP-independent periplasmic) family. An fcbT1T2T3 knockout mutant shows a much slower growth rate on 4-chlorobenzoate compared to the wild type. 4-Chlorobenzoate is transported into the wild-type strain five times faster than into the fcbT1T2T3 knockout mutant. Transport of 4-chlorobenzoate shows significant inhibition by 4-bromo-, 4-iodo-, and 4-fluorobenzoate and mild inhibition by 3-chlorobenzoate, 2-chlorobenzoate, 4-hydroxybenzoate, 3-hydroxybenzoate, and benzoate. Uptake of 4-chlorobenzoate is significantly inhibited by ionophores which collapse the proton motive force.

* Corresponding author. Mailing address: Biotech Center, Foran Hall, 59 Dudley Rd., Cook College, Rutgers University, New Brunswick, NJ 08901-8520. Phone: (732) 932-8165, ext. 210. Fax: (732) 932-0312. E-mail: zylstra{at}aesop.rutgers.edu.

{triangledown} Published ahead of print on 13 October 2006.

Journal of Bacteriology, December 2006, p. 8407-8412, Vol. 188, No. 24
0021-9193/06/$08.00+0     doi:10.1128/JB.00880-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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