This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vivas, E.
Right arrow Articles by Downs, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vivas, E.
Right arrow Articles by Downs, D. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2006, p. 1175-1179, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.1175-1179.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Salmonella enterica Strains Lacking the Frataxin Homolog CyaY Show Defects in Fe-S Cluster Metabolism In Vivo

E. Vivas, E. Skovran,{dagger} and D. M. Downs*

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 2 September 2005/ Accepted 11 November 2005

In Salmonella enterica, the isc operon contains genes necessary for the synthesis of Fe-S clusters and strains lacking this operon have severe defects in a variety of cellular processes. Other cellular loci that impact Fe-S cluster synthesis to a lesser extent have been described. The cyaY locus encodes a frataxin homolog, and it is shown here that lesions in this locus affect Fe-S cluster metabolism. When present in combination with other lesions, mutations in cyaY can result in a strain with more severe defects than those lacking the isc locus.

* Corresponding author. Mailing address: Department of Bacteriology, University of Wisconsin—Madison, 420 Henry Mall, Madison, WI 53706-1502. Phone: (608) 265-4630. Fax: (608) 890-0785. E-mail: downs{at}bact.wisc.edu.

{dagger} Present address: University of Washington, Department of Chemical Engineering, Seattle, WA 98195.

Journal of Bacteriology, February 2006, p. 1175-1179, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.1175-1179.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, H., Gakh, O., Smith, D. Y. IV, Isaya, G. (2009). Oligomeric Yeast Frataxin Drives Assembly of Core Machinery for Mitochondrial Iron-Sulfur Cluster Synthesis. J. Biol. Chem. 284: 21971-21980 [Abstract] [Full Text]  
  • Boyd, J. M., Sondelski, J. L., Downs, D. M. (2009). Bacterial ApbC Protein Has Two Biochemical Activities That Are Required for in Vivo Function. J. Biol. Chem. 284: 110-118 [Abstract] [Full Text]  
  • Thorgersen, M. P., Downs, D. M. (2009). Oxidative stress and disruption of labile iron generate specific auxotrophic requirements in Salmonella enterica. Microbiology 155: 295-304 [Abstract] [Full Text]  
  • Thorgersen, M. P., Downs, D. M. (2008). Analysis of yggX and gshA Mutants Provides Insights into the Labile Iron Pool in Salmonella enterica. J. Bacteriol. 190: 7608-7613 [Abstract] [Full Text]  
  • Boyd, J. M., Lewis, J. A., Escalante-Semerena, J. C., Downs, D. M. (2008). Salmonella enterica Requires ApbC Function for Growth on Tricarballylate: Evidence of Functional Redundancy between ApbC and IscU. J. Bacteriol. 190: 4596-4602 [Abstract] [Full Text]  
  • Ayala-Castro, C., Saini, A., Outten, F. W. (2008). Fe-S Cluster Assembly Pathways in Bacteria. Microbiol. Mol. Biol. Rev. 72: 110-125 [Abstract] [Full Text]  
  • Thorgersen, M. P., Downs, D. M. (2007). Cobalt Targets Multiple Metabolic Processes in Salmonella enterica. J. Bacteriol. 189: 7774-7781 [Abstract] [Full Text]  
  • Ding, H., Yang, J., Coleman, L. C., Yeung, S. (2007). Distinct Iron Binding Property of Two Putative Iron Donors for the Iron-Sulfur Cluster Assembly: IscA AND THE BACTERIAL FRATAXIN ORTHOLOG CyaY UNDER PHYSIOLOGICAL AND OXIDATIVE STRESS CONDITIONS. J. Biol. Chem. 282: 7997-8004 [Abstract] [Full Text]  
  • Layer, G., Ollagnier-de Choudens, S., Sanakis, Y., Fontecave, M. (2006). Iron-Sulfur Cluster Biosynthesis: CHARACTERIZATION OF ESCHERICHIA COLI CYaY AS AN IRON DONOR FOR THE ASSEMBLY OF [2Fe-2S] CLUSTERS IN THE SCAFFOLD IscU. J. Biol. Chem. 281: 16256-16263 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»