This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Froelich, J. M.
Right arrow Articles by Wall, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Froelich, J. M.
Right arrow Articles by Wall, D.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2006, p. 1180-1183, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.1180-1183.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A pmrA Constitutive Mutant Sensitizes Escherichia coli to Deoxycholic Acid

Jamie M. Froelich, Khoa Tran, and Daniel Wall*

Anadys Pharmaceuticals, Inc., San Diego, Calif

Received 22 August 2005/ Accepted 10 November 2005

An Escherichia coli mutant was isolated and shown to be polymyxin B resistant. Mapping and sequence analysis revealed a missense mutation at codon 53 within the pmrA (basR) gene that results in a G-to-V substitution. Fusions of promoters from the pmrC, yibD, and pmrH genes with the lacZ reporter showed that they were constitutively expressed in pmrA53 cells. In pmrA+ strains, these promoters were induced by iron and zinc, while a {Delta}pmrA mutation blocked induction. The PmrA regulon regulates genes whose products remodel the composition and charge of lipid A and hence the barrier properties of the outer membrane. Along these lines, the pmrA53 mutant was also found to be hypersensitive to the anionic bile detergent deoxycholic acid.

* Corresponding author. Mailing address: Department of Microbiology, Anadys Pharmaceuticals, Inc., 3115 Merryfield Row, San Diego, CA 92121. Phone: (858) 530-3711. Fax: (858) 530-3644. E-mail: dwall{at}anadyspharma.com.

Journal of Bacteriology, February 2006, p. 1180-1183, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.1180-1183.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»