Journal of Bacteriology, February 2006, p. 1196-1198, Vol. 188, No. 3
0021-9193/06/$08.00+0 doi:10.1128/JB.188.3.1196-1198.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Transcriptional Slippage in mxiE Controls Transcription and Translation of the Downstream mxiD Gene, Which Encodes a Component of the Shigella flexneri Type III Secretion Apparatus
Christophe Penno and
Claude Parsot*
Unité de Pathogénie Microbienne Moléculaire, INSERM U389, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France
Received 16 September 2005/
Accepted 10 November 2005
The Shigella flexneri transcription activator MxiE is produced by transcriptional slippage from two overlapping open reading frames. By using plasmids encoding a mxiD-lacZ translational fusion, we showed that transcriptional slippage in mxiE influences both transcription and translation of the downstream mxiD gene encoding an essential component of the type III secretion apparatus.
* Corresponding author. Mailing address: Unité de Pathogénie Microbienne Moléculaire, INSERM U389, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: 33 01 40 61 38 42. Fax: 33 01 45 68 89 53. E-mail: cparsot{at}pasteur.fr.
Journal of Bacteriology, February 2006, p. 1196-1198, Vol. 188, No. 3
0021-9193/06/$08.00+0 doi:10.1128/JB.188.3.1196-1198.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Copyright © 2006 by the American Society for Microbiology. All rights reserved.