This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Qiu, X. Articles by Tiedje, J. M. Search for Related Content PubMed PubMed Citation Articles by Qiu, X. Articles by Tiedje, J. M.

Transcriptome Analysis Applied to Survival of Shewanella oneidensis MR-1 Exposed to Ionizing Radiation

Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824,¹ Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814,² National Institutes of Health, Bethesda, Maryland 20894,³ Environmental Science Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831,⁴ Department of Plant Pathology, Michigan State University, East Lansing, Michigan 48824⁵

Received 9 July 2005/ Accepted 15 November 2005

The ionizing radiation (IR) dose that yields 20% survival (D₂₀) of Shewanella oneidensis MR-1 is lower by factors of 20 and 200 than those for Escherichia coli and Deinococcus radiodurans, respectively. Transcriptome analysis was used to identify the genes of MR-1 responding to 40 Gy (D₂₀). We observed the induction of 170 genes and repression of 87 genes in MR-1 during a 1-h recovery period after irradiation. The genomic response of MR-1 to IR is very similar to its response to UV radiation (254 nm), which included induction of systems involved in DNA repair and prophage synthesis and the absence of differential regulation of tricarboxylic acid cycle activity, which occurs in IR-irradiated D. radiodurans. Furthermore, strong induction of genes encoding antioxidant enzymes in MR-1 was observed. DNA damage may not be the principal cause of high sensitivity to IR, considering that MR-1 carries genes encoding a complex set of DNA repair systems and 40 Gy IR induces less than one double-strand break in its genome. Instead, a combination of oxidative stress, protein damage, and prophage-mediated cell lysis during irradiation and recovery might underlie this organism's great sensitivity to IR.

This article has been cited by other articles:

• Gladyshev, E., Meselson, M. (2008). Extreme resistance of bdelloid rotifers to ionizing radiation. Proc. Natl. Acad. Sci. USA 105: 5139-5144 [Abstract] [Full Text]  
• Moeller, R., Setlow, P., Horneck, G., Berger, T., Reitz, G., Rettberg, P., Doherty, A. J., Okayasu, R., Nicholson, W. L. (2008). Roles of the Major, Small, Acid-Soluble Spore Proteins and Spore-Specific and Universal DNA Repair Mechanisms in Resistance of Bacillus subtilis Spores to Ionizing Radiation from X Rays and High-Energy Charged-Particle Bombardment. J. Bacteriol. 190: 1134-1140 [Abstract] [Full Text]  
• Yang, C., Rodionov, D. A., Li, X., Laikova, O. N., Gelfand, M. S., Zagnitko, O. P., Romine, M. F., Obraztsova, A. Y., Nealson, K. H., Osterman, A. L. (2006). Comparative Genomics and Experimental Characterization of N-Acetylglucosamine Utilization Pathway of Shewanella oneidensis. J. Biol. Chem. 281: 29872-29885 [Abstract] [Full Text]  
• Chourey, K., Thompson, M. R., Morrell-Falvey, J., VerBerkmoes, N. C., Brown, S. D., Shah, M., Zhou, J., Doktycz, M., Hettich, R. L., Thompson, D. K. (2006). Global Molecular and Morphological Effects of 24-Hour Chromium(VI) Exposure on Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 72: 6331-6344 [Abstract] [Full Text]