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The Vibrio harveyi GTPase CgtA_V Is Essential and Is Associated with the 50S Ribosomal Subunit

Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, Michigan 48109,¹ Department of Molecular Biology, University of Gdansk, Kadki 24, 80-822 Gdansk, Poland²

Received 14 September 2005/ Accepted 28 October 2005

It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtA_V is not essential. Here we show that cgtA_V was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtA_V did not result in cell elongation. CgtA_V is associated with the large ribosomal particle. In light of our results, we predict that the V. harveyi CgtA_V protein plays a similar essential role to that seen for Obg/CgtA proteins in other bacteria.

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