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Journal of Bacteriology, February 2006, p. 934-940, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.934-940.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Effect of Nitrogen Source on Cyanophycin Synthesis in Synechocystis sp. Strain PCC 6308

Nancy H. Kolodny,1 Deborah Bauer,2 Kerstyn Bryce,2 Kristen Klucevsek,2 Amy Lane,1 Lea Medeiros,1 Wendy Mercer,1 Sogole Moin,2 Deborah Park,1 Juliette Petersen,1 Julie Wright,2 Courtney Yuen,1 Adele J. Wolfson,1 and Mary M. Allen2*

Departments of Chemistry,1 Biological Sciences, Wellesley College, Wellesley, Massachusetts 024812

Received 28 June 2005/ Accepted 2 November 2005

Experiments were carried out to examine the effects of nitrogen source on nitrogen incorporation into cyanophycin during nitrogen limitation and repletion, both with or without inhibition of protein synthesis, in cyanobacteria grown on either nitrate or ammonium. The use of nitrate and ammonium, 14N labeled in the growth medium and 15N labeled in the repletion medium, allows the determination of the source of nitrogen in cyanophycin using proton nuclear magnetic resonance spectroscopy. The data suggest that nitrogen from both the breakdown of cellular protein (14N) and directly from the medium (15N) is incorporated into cyanophycin. Nitrogen is incorporated into cyanophycin at different rates and to different extents, depending on the source of nitrogen (ammonium or nitrate) and whether the cells are first starved for nitrogen. These differences appear to be related to the activity of nitrate reductase in cells and to the possible expression of cyanophycin synthetase during nitrogen starvation.

* Corresponding author. Mailing address: Department of Biological Sciences, Wellesley College, 106 Central St., Wellesley, MA 02481. Phone: (781) 283-3068. Fax: (781) 283-3642. E-mail: mallen{at}wellesley.edu.

Journal of Bacteriology, February 2006, p. 934-940, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.934-940.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schriek, S., Kahmann, U., Staiger, D., Pistorius, E. K., Michel, K.-P. (2009). Detection of an L-amino acid dehydrogenase activity in Synechocystis sp. PCC 6803. J Exp Bot 60: 1035-1046 [Abstract] [Full Text]  


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