This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zeng, L.
Right arrow Articles by Burne, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeng, L.
Right arrow Articles by Burne, R. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2006, p. 941-949, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.941-949.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Characterization of cis-Acting Sites Controlling Arginine Deiminase Gene Expression in Streptococcus gordonii

Lin Zeng, Yiqian Dong,{ddagger} and Robert A. Burne*

Department of Oral Biology, University of Florida, Gainesville, Florida

Received 25 August 2005/ Accepted 7 November 2005

The arginine deiminase system (ADS) is responsible for the production of ornithine, CO2, ammonia, and ATP from arginine. The ADS of the oral bacterium Streptococcus gordonii plays major roles in physiologic homeostasis, acid tolerance, and oral biofilm ecology. To further our understanding of the transcriptional regulation of the ADS (arc) operon, the binding of the ArcR transcriptional activator, which governs expression of the ADS in response to arginine, was investigated by DNase I protection and gel mobility shift assays. An ArcR binding sequence was found that was 27 bp in length and had little sequence similarity to binding sites of other arginine metabolism regulators. The presence of arginine at physiologically relevant concentrations enhanced the binding of ArcR to its target. Using cat fusions, various deletion and substitution mutations within the putative ArcR footprint were shown to cause dramatic reductions in expression from the arcA promoter in vivo, confirming that the 27-bp sequence is required for optimal expression and induction of the ADS by arginine. Mutation of two putative catabolite response elements (CREs) within the arc promoter region showed that both CREs contribute to catabolite repression. A thorough understanding of the regulation of the ADS in S. gordonii and related organisms is needed to develop ways to exploit arginine catabolism for the control of oral diseases. Identification of the ArcR and CcpA binding sites lays the foundation for a more complete understanding of the complex interactions of multiple regulatory proteins with elements in the arc promoter region.

* Corresponding author. Mailing address: Department of Oral Biology, University of Florida, 1600 S.W. Archer Rd., Gainesville, FL 32610-0424. Phone: (352) 392-0011. Fax: (352) 392-7357. E-mail: rburne{at}dental.ufl.edu.

{ddagger} Present address: University of Rochester Eastman Dental Center, 625 Elmwood Ave., Rochester, NY 14620.

Journal of Bacteriology, February 2006, p. 941-949, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.941-949.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zeng, L., Burne, R. A. (2009). Transcriptional Regulation of the Cellobiose Operon of Streptococcus mutans. J. Bacteriol. 191: 2153-2162 [Abstract] [Full Text]  
  • Lin, X., Lamont, R. J., Wu, J., Xie, H. (2008). Role of Differential Expression of Streptococcal Arginine Deiminase in Inhibition of fimA Expression in Porphyromonas gingivalis. J. Bacteriol. 190: 4367-4371 [Abstract] [Full Text]  
  • Jakubovics, N. S., Gill, S. R., Iobst, S. E., Vickerman, M. M., Kolenbrander, P. E. (2008). Regulation of Gene Expression in a Mixed-Genus Community: Stabilized Arginine Biosynthesis in Streptococcus gordonii by Coaggregation with Actinomyces naeslundii. J. Bacteriol. 190: 3646-3657 [Abstract] [Full Text]  
  • Abranches, J., Nascimento, M. M., Zeng, L., Browngardt, C. M., Wen, Z. T., Rivera, M. F., Burne, R. A. (2008). CcpA Regulates Central Metabolism and Virulence Gene Expression in Streptococcus mutans. J. Bacteriol. 190: 2340-2349 [Abstract] [Full Text]  
  • Xie, H., Lin, X., Wang, B.-Y., Wu, J., Lamont, R. J. (2007). Identification of a signalling molecule involved in bacterial intergeneric communication. Microbiology 153: 3228-3234 [Abstract] [Full Text]  
  • Makhlin, J., Kofman, T., Borovok, I., Kohler, C., Engelmann, S., Cohen, G., Aharonowitz, Y. (2007). Staphylococcus aureus ArcR Controls Expression of the Arginine Deiminase Operon. J. Bacteriol. 189: 5976-5986 [Abstract] [Full Text]  
  • Kaufman, G. E., Yother, J. (2007). CcpA-Dependent and -Independent Control of Beta-Galactosidase Expression in Streptococcus pneumoniae Occurs via Regulation of an Upstream Phosphotransferase System-Encoding Operon. J. Bacteriol. 189: 5183-5192 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»