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Journal of Bacteriology, February 2006, p. 1540-1550, Vol. 188, No. 4
0021-9193/06/$08.00+0     doi:10.1128/JB.188.4.1540-1550.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Influence of CrgA on Assembly of the Cell Division Protein FtsZ during Development of Streptomyces coelicolor

Ricardo Del Sol,1 Jonathan G. L. Mullins,1 Nina Grantcharova,2 Klas Flärdh,3 and Paul Dyson1*

Institute of Life Science, School of Medicine, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, United Kingdom,1 Department of Cell and Molecular Biology, Uppsala University, BMC, Box 596, SE-751 24 Uppsala, Sweden,2 Department of Cell and Organism Biology, Lund University, Sölvegatan 35, SE-223 62 Lund, Sweden3

Received 17 October 2005/ Accepted 22 November 2005

The product of the crgA gene of Streptomyces coelicolor represents a novel family of small proteins. A single orthologous gene is located close to the origin of replication of all fully sequenced actinomycete genomes and borders a conserved gene cluster implicated in cell growth and division. In S. coelicolor, CrgA is important for coordinating growth and cell division in sporogenic hyphae. In this study, we demonstrate that CrgA is an integral membrane protein whose peak expression is coordinated with the onset of development of aerial hyphae. The protein localizes to discrete foci away from growing hyphal tips. Upon overexpression, CrgA localizes to apical syncytial cells of aerial hyphae and inhibits the formation of productive cytokinetic rings of the bacterial tubulin homolog FtsZ, leading to proteolytic turnover of this major cell division determinant. In the absence of known prokaryotic cell division inhibitors in actinomycetes, CrgA may have an important conserved function influencing Z-ring formation in these bacteria.

* Corresponding author. Mailing address: Institute of Life Science, School of Medicine, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, United Kingdom. Phone: 44-1792 295667. Fax: 44-1792 295447. E-mail: p.j.dyson{at}swansea.ac.uk.

Journal of Bacteriology, February 2006, p. 1540-1550, Vol. 188, No. 4
0021-9193/06/$08.00+0     doi:10.1128/JB.188.4.1540-1550.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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