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Journal of Bacteriology, February 2006, p. 1620-1627, Vol. 188, No. 4
0021-9193/06/$08.00+0     doi:10.1128/JB.188.4.1620-1627.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Apyrase, the Product of the Virulence Plasmid-Encoded phoN2 (apy) Gene of Shigella flexneri, Is Necessary for Proper Unipolar IcsA Localization and for Efficient Intercellular Spread{dagger}

D. Santapaola,1 F. Del Chierico,2 A. Petrucca,3 S. Uzzau,4 M. Casalino,5 B. Colonna,6 R. Sessa,2 F. Berlutti,2 and M. Nicoletti1*

Dipartimento di Scienze Biomediche, Università "G. D'Annunzio," 66100 Chieti,1 Dipartimento di Scienze Biomediche, Università degli Studi di Sassari, 07100 Sassari,4 Dipartimento di Biologia, Università di "Roma Tre," 00146 Rome,5 Dipartimento di Scienze di Sanità Pubblica,2 Laboratorio di Microbiologia Clinica, II Facoltà di Medicina e Chirurgia, Ospedale "Sant'Andrea," 00189 Rome,3 Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma "La Sapienza," 00185 Rome, Italy6

Received 15 September 2005/ Accepted 24 November 2005

The role in virulence of the Shigella flexneri ospB-phoN2 operon has been evaluated. Here we confirm that OspB is an effector and show that apyrase, the product of phoN2, may be a virulence factor, since it is required for efficient intercellular spreading. Apyrase may be important in a deoxynucleoside triphosphate-hydrolyzing activity-independent manner, suggesting that it may act as an interaction partner in the process of IcsA localization.

* Corresponding author. Mailing address: Dipartimento di Scienze Biomediche, Sezione di Microbiologia, Università "G. D'Annunzio," Via dei Vestini, 31, 66100 Chieti, Italy. Phone: 39-06-49914657. Fax: 39-06-49914626. E-mail: mauro.nicoletti{at}uniroma1.it.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, February 2006, p. 1620-1627, Vol. 188, No. 4
0021-9193/06/$08.00+0     doi:10.1128/JB.188.4.1620-1627.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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