This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Luong, T. T. Articles by Lee, C. Y. Search for Related Content PubMed PubMed Citation Articles by Luong, T. T. Articles by Lee, C. Y.

Transcription Profiling of the mgrA Regulon in Staphylococcus aureus

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205,¹ Department of Microbiology and Pathology, University of Nebraska Medical Center, Omaha, Nebraska 68198,² Wyeth Research, Pearl River, New York,³ Wyeth Research, Cambridge, Massachusetts⁴

Received 26 August 2005/ Accepted 8 December 2005

MgrA has been shown to affect multiple Staphylococcus aureus genes involved in virulence and antibiotic resistance. To comprehensively identify the target genes regulated by mgrA, we employed a microarray method to analyze the transcription profiles of S. aureus Newman, its isogeneic mgrA mutant, and an MgrA-overproducing derivative. We compared genes that were differentially expressed at exponential or early stationary growth phases. Our results showed that MgrA affected an impressive number of genes, 175 of which were positively regulated and 180 of which were negatively regulated in an mgrA-specific manner. The target genes included all functional categories. The microarray results were validated by real-time reverse transcription-PCR quantitation of a set of selected genes from different functional categories. Our data also indicate that mgrA regulates virulence factors in a fashion analogous to that of the accessory gene regulatory locus (agr). Accordingly, exoproteins are upregulated and surface proteins are downregulated by the regulator, suggesting that mgrA may function in concert with agr. The fact that a large number of genes are regulated by mgrA implies that MgrA is a major global regulator in S. aureus.

This article has been cited by other articles:

• Guo, B., Wang, Y., Shi, F., Barton, Y.-W., Plummer, P., Reynolds, D. L., Nettleton, D., Grinnage-Pulley, T., Lin, J., Zhang, Q. (2008). CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo. J. Bacteriol. 190: 1879-1890 [Abstract] [Full Text]  
• Handke, L. D., Rogers, K. L., Olson, M. E., Somerville, G. A., Jerrells, T. J., Rupp, M. E., Dunman, P. M., Fey, P. D. (2008). Staphylococcus epidermidis saeR Is an Effector of Anaerobic Growth and a Mediator of Acute Inflammation. Infect. Immun. 76: 141-152 [Abstract] [Full Text]  
• Antignac, A., Sieradzki, K., Tomasz, A. (2007). Perturbation of Cell Wall Synthesis Suppresses Autolysis in Staphylococcus aureus: Evidence for Coregulation of Cell Wall Synthetic and Hydrolytic Enzymes. J. Bacteriol. 189: 7573-7580 [Abstract] [Full Text]  
• DeMarco, C. E., Cushing, L. A., Frempong-Manso, E., Seo, S. M., Jaravaza, T. A. A., Kaatz, G. W. (2007). Efflux-Related Resistance to Norfloxacin, Dyes, and Biocides in Bloodstream Isolates of Staphylococcus aureus. Antimicrob. Agents Chemother. 51: 3235-3239 [Abstract] [Full Text]  
• Meier, S., Goerke, C., Wolz, C., Seidl, K., Homerova, D., Schulthess, B., Kormanec, J., Berger-Bachi, B., Bischoff, M. (2007). {sigma}B and the {sigma}B-Dependent arlRS and yabJ-spoVG Loci Affect Capsule Formation in Staphylococcus aureus. Infect. Immun. 75: 4562-4571 [Abstract] [Full Text]  
• Boisset, S., Geissmann, T., Huntzinger, E., Fechter, P., Bendridi, N., Possedko, M., Chevalier, C., Helfer, A. C., Benito, Y., Jacquier, A., Gaspin, C., Vandenesch, F., Romby, P. (2007). Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism. Genes Dev. 21: 1353-1366 [Abstract] [Full Text]  
• Zheng, L., Yu, C., Bayles, K., Lasa, I., Ji, Y. (2007). Conditional Mutation of an Essential Putative Glycoprotease Eliminates Autolysis in Staphylococcus aureus. J. Bacteriol. 189: 2734-2742 [Abstract] [Full Text]  
• Riordan, J. T., Muthaiyan, A., Van Voorhies, W., Price, C. T., Graham, J. E., Wilkinson, B. J., Gustafson, J. E. (2007). Response of Staphylococcus aureus to Salicylate Challenge. J. Bacteriol. 189: 220-227 [Abstract] [Full Text]  
• Cassat, J., Dunman, P. M., Murphy, E., Projan, S. J., Beenken, K. E., Palm, K. J., Yang, S.-J., Rice, K. C., Bayles, K. W., Smeltzer, M. S. (2006). Transcriptional profiling of a Staphylococcus aureus clinical isolate and its isogenic agr and sarA mutants reveals global differences in comparison to the laboratory strain RN6390.. Microbiology 152: 3075-3090 [Abstract] [Full Text]  
• Luong, T. T., Lee, C. Y. (2006). The arl locus positively regulates Staphylococcus aureus type 5 capsule via an mgrA-dependent pathway.. Microbiology 152: 3123-3131 [Abstract] [Full Text]  
• Renzoni, A., Barras, C., Francois, P., Charbonnier, Y., Huggler, E., Garzoni, C., Kelley, W. L., Majcherczyk, P., Schrenzel, J., Lew, D. P., Vaudaux, P. (2006). Transcriptomic and Functional Analysis of an Autolysis-Deficient, Teicoplanin-Resistant Derivative of Methicillin-Resistant Staphylococcus aureus.. Antimicrob. Agents Chemother. 50: 3048-3061 [Abstract] [Full Text]  
• Manna, A. C., Cheung, A. L. (2006). Expression of SarX, a Negative Regulator of agr and Exoprotein Synthesis, Is Activated by MgrA in Staphylococcus aureus.. J. Bacteriol. 188: 4288-4299 [Abstract] [Full Text]