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Journal of Bacteriology, March 2006, p. 2014-2019, Vol. 188, No. 5
0021-9193/06/$08.00+0     doi:10.1128/JB.188.5.2014-2019.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of an ATP-Dependent Hexokinase with Broad Substrate Specificity from the Hyperthermophilic Archaeon Sulfolobus tokodaii

Hiroshi Nishimasu, Shinya Fushinobu, Hirofumi Shoun, and Takayoshi Wakagi*

Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

Received 4 October 2005/ Accepted 16 December 2005

As a new member of the glucose-phosphorylating enzymes, the ATP-dependent hexokinase from the hyperthermophilic crenarchaeon Sulfolobus tokodaii was purified, identified, and characterized. Our results revealed that the enzyme differs from other known enzymes in primary structure and its broad substrate specificity for both phosphoryl donors and acceptors.

* Corresponding author. Mailing address: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Phone: 81-3-5841-5152. Fax: 81-3-5841-5152. E-mail: atwakag{at}mail.ecc.u-tokyo.ac.jp.

Journal of Bacteriology, March 2006, p. 2014-2019, Vol. 188, No. 5
0021-9193/06/$08.00+0     doi:10.1128/JB.188.5.2014-2019.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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