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Journal of Bacteriology, March 2006, p. 2280-2284, Vol. 188, No. 6
0021-9193/06/$08.00+0     doi:10.1128/JB.188.6.2280-2284.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Harpin Mediates Cell Aggregation in Erwinia chrysanthemi 3937

Mee-Ngan Yap,1 Clemencia M. Rojas,2 Ching-Hong Yang,3 and Amy O. Charkowski1*

Department of Plant Pathology, 1630 Linden Dr., University of Wisconsin—Madison, Madison, Wisconsin 53706,1 Department of Molecular Microbiology, Washington University School of Medicine, 660 S. Euclid Ave., Campus Box 8230, St. Louis, Missouri 63110,2 Department of Biological Sciences, 181 Lapham Hall, 3209 N. Maryland Ave., University of Wisconsin—Milwaukee, Milwaukee, Wisconsin 532113

Received 3 June 2005/ Accepted 4 January 2006

The hypersensitive response elicitor harpin (HrpN) of soft rot pathogen Erwinia chrysanthemi strains 3937 and EC16 is secreted via the type III secretion system and remains cell surface bound. Strain 3937 HrpN is essential for cell aggregation, but the C-terminal one-third of the protein is not required for aggregative activity.


* Corresponding author. Mailing address: Department of Plant Pathology, 1630 Linden Dr., University of Wisconsin—Madison, Madison, WI 53706. Phone: (608) 262-7911. Fax: (608) 263-2626. E-mail: amyc{at}plantpath.wisc.edu.


Journal of Bacteriology, March 2006, p. 2280-2284, Vol. 188, No. 6
0021-9193/06/$08.00+0     doi:10.1128/JB.188.6.2280-2284.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




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