This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maamar, H.
Right arrow Articles by Tardif, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maamar, H.
Right arrow Articles by Tardif, C.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2006, p. 2614-2624, Vol. 188, No. 7
0021-9193/06/$08.00+0     doi:10.1128/JB.188.7.2614-2624.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Transcriptional Analysis of the cip-cel Gene Cluster from Clostridium cellulolyticum

Hédia Maamar ,1,{dagger},{ddagger} Laetitia Abdou,1,{dagger} Céline Boileau,1 Odile Valette,1 and Chantal Tardif1,2*

Laboratoire de Bioénergétique et Ingénierie des Protéines, UPR 9036-CNRS, 13402 Marseille Cedex 20,1 Université de Provence, 13331 Marseille Cedex 03 France2

Received 13 September 2005/ Accepted 16 January 2006

Twelve genes encoding key components of Clostridium cellulolyticum cellulosomes are clustered. Among them, the first, second, and fifth genes encode the assembly factor CipC and the two major cellulases Cel48F and Cel9E, respectively. Cellulolytic clones were selected from the noncellulolytic cipC insertional mutant trans-complemented with a cipC expression vector, in which one homologous recombination event between the 3' end of the chromosomal cipC gene and the plasmidic cipC gene has restored the cluster continuity. The absence of the enzymes encoded by the cluster in the cipC mutant was thus only due to a strong polar effect, indicating that all genes were transcriptionally linked. Two large transcripts were detected in cellulose-grown cells by Northern hybridization: a 14-kb messenger which carries the cipC-cel48F-cel8C-cel9G-cel9E coding sequences and, in a smaller amount, a 12-kb messenger which carries the genes located in the 3' part of the cluster. Four smaller transcripts were found in large amounts: a cipC-cel48F bicistronic one and three monocistronic ones, cipC, cel48F, and cel9E. The cipC-cel48F and cel48F messengers were shown to be stable. Analysis by reverse transcription-PCR suggested transcriptional linkage of all of the open reading frames. The production of a primary very large transcript covering the entire cluster was hypothesized. Primer extension analysis has identified two putative transcriptional start sites located 638/637 and 194 nucleotides upstream of the cipC translational start. The processing of the primary transcript would lead to the production of several secondary messengers displaying different stabilities, contributing to fine tuning of expression of individual genes of the operon.

* Corresponding author. Mailing address: BIP-CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Phone: (33) 4 91 16 43 40. Fax: (33) 4 91 71 33 21. E-mail: tardif{at}ibsm.cnrs-mrs.fr.

{dagger} H.M. and L.A. contributed equally to this work.

{ddagger} Present address: Public Health Research Institute, Room W410A, 225 Warren Street, Newark, NJ 07103.

Journal of Bacteriology, April 2006, p. 2614-2624, Vol. 188, No. 7
0021-9193/06/$08.00+0     doi:10.1128/JB.188.7.2614-2624.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zverlov, V. V., Klupp, M., Krauss, J., Schwarz, W. H. (2008). Mutations in the Scaffoldin Gene, cipA, of Clostridium thermocellum with Impaired Cellulosome Formation and Cellulose Hydrolysis: Insertions of a New Transposable Element, IS1447, and Implications for Cellulase Synergism on Crystalline Cellulose. J. Bacteriol. 190: 4321-4327 [Abstract] [Full Text]  
  • Abdou, L., Boileau, C., de Philip, P., Pages, S., Fierobe, H.-P., Tardif, C. (2008). Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element. J. Bacteriol. 190: 1499-1506 [Abstract] [Full Text]  
  • Blouzard, J.-C., Bourgeois, C., de Philip, P., Valette, O., Belaich, A., Tardif, C., Belaich, J.-P., Pages, S. (2007). Enzyme Diversity of the Cellulolytic System Produced by Clostridium cellulolyticum Explored by Two-Dimensional Analysis: Identification of Seven Genes Encoding New Dockerin-Containing Proteins. J. Bacteriol. 189: 2300-2309 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»