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Journal of Bacteriology, April 2006, p. 2636-2645, Vol. 188, No. 7
0021-9193/06/$08.00+0     doi:10.1128/JB.188.7.2636-2645.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Response Regulator CroR Modulates Expression of the Secreted Stress-Induced SalB Protein in Enterococcus faecalis

Cécile Muller, Yoann Le Breton,^, Thierry Morin, Abdellah Benachour, Yanick Auffray, and Alain Rincé^*

USC INRA 2017 Microbiologie de l'Environnement, EA 956, IRBA, Université de Caen, 14032 CAEN cedex, France

Received 21 October 2005/ Accepted 18 January 2006

The Enterococcus faecalis two-component signal transduction system CroRS, also referred as the RR-HK05 pair, is required for intrinsic ß-lactam resistance (Y. R. Comenge, R. Quintiliani, Jr., L. Li, L. Dubost, J. P. Brouard, J. E. Hugonnet, and M. Arthur, J. Bacteriol. 185:7184-7192, 2003) and is also suspected to be involved in the expression of salB (previously referred to as sagA), a gene important for resistance to environmental stress and cell morphology (Y. Le Breton, G. Boël, A. Benachour, H. Prévost, Y. Auffray, and A. Rincé, Environ. Microbiol. 5:329-337, 2003). In this report, we provide genetic and biochemical evidence that salB encodes a secreted protein that is expressed from a monocistronic stress-inducible operon. Consistent with CroR being a direct transcriptional activator of the salB expression, CroR was found to bind to the salB promoter region in electrophoretic mobility shift assays. Interestingly, we provide evidence that SalB does not play a role in the intrinsic ß-lactam resistance associated with CroRS. We also show that the CroRS system is able to regulate its own expression. The sequence of the CroRS binding site in the salB and croR promoter regions was determined using DNase I footprinting assays.

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* Corresponding author. Mailing address: USC INRA 2017 Microbiologie de l'Environnement, EA 956, IRBA, Université de Caen, 14032 CAEN cedex, France. Phone: (33) 2 31 56 55 23. Fax: (33) 2 31 56 53 11. E-mail: alain.rince{at}unicaen.fr.

C.M. and Y.L.B. contributed equally to this work.

Present address: Dept. of Microbiology and Immunology, University of Texas Health Science Center, San Antonio, TX.

Present address: Plate-Forme Technologique d'Evreux, 55 rue Saint Germain, 27 000 EVREUX, France.

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Journal of Bacteriology, April 2006, p. 2636-2645, Vol. 188, No. 7
0021-9193/06/$08.00+0     doi:10.1128/JB.188.7.2636-2645.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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• Le Breton, Y., Muller, C., Auffray, Y., Rince, A. (2007). New Insights into the Enterococcus faecalis CroRS Two-Component System Obtained Using a Differential-Display Random Arbitrarily Primed PCR Approach. Appl. Environ. Microbiol. 73: 3738-3741 [Abstract] [Full Text]