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Journal of Bacteriology, April 2006, p. 2812-2820, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.2812-2820.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Differences in Resolution of mwr-Containing Plasmid Dimers Mediated by the Klebsiella pneumoniae and Escherichia coli XerC Recombinases: Potential Implications in Dissemination of Antibiotic Resistance Genes

Duyen Bui,1,2 Judianne Ramiscal,1,2 Sonia Trigueros,2 Jason S. Newmark,1,2 Albert Do,1 David J. Sherratt,2 and Marcelo E. Tolmasky1,2*

Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, California 92834-6850,1 Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom2

Received 18 November 2005/ Accepted 3 February 2006

Xer-mediated dimer resolution at the mwr site of the multiresistance plasmid pJHCMW1 is osmoregulated in Escherichia coli containing either the Escherichia coli Xer recombination machinery or Xer recombination elements from K. pneumoniae. In the presence of K. pneumoniae XerC (XerCKp), the efficiency of recombination is lower than that in the presence of the E. coli XerC (XerCEc) and the level of dimer resolution is insufficient to stabilize the plasmid, even at low osmolarity. This lower efficiency of recombination at mwr is observed in the presence of E. coli or K. pneumoniae XerD proteins. Mutagenesis experiments identified a region near the N terminus of XerCKp responsible for the lower level of recombination catalyzed by XerCKp at mwr. This region encompasses the second half of the predicted {alpha}-helix B and the beginning of the predicted {alpha}-helix C. The efficiencies of recombination at other sites such as dif or cer in the presence of XerCKp or XerCEc are comparable. Therefore, XerCKp is an active recombinase whose action is impaired on the mwr recombination site. This characteristic may result in restriction of the host range of plasmids carrying this site, a phenomenon that may have important implications in the dissemination of antibiotic resistance genes.

* Corresponding author. Mailing address: Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA 92834-6850. Phone: (714) 278-5263. Fax: (714) 278-3426. E-mail: mtolmasky{at}fullerton.edu.

Journal of Bacteriology, April 2006, p. 2812-2820, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.2812-2820.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Trigueros, S., Tran, T., Sorto, N., Newmark, J., Colloms, S. D., Sherratt, D. J., Tolmasky, M. E. (2009). mwr Xer site-specific recombination is hypersensitive to DNA supercoiling. Nucleic Acids Res 37: 3580-3587 [Abstract] [Full Text]  


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