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Journal of Bacteriology, April 2006, p. 2836-2844, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.2836-2844.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of Lactaldehyde Dehydrogenase in Methanocaldococcus jannaschii and Its Involvement in Production of Lactate for F420 Biosynthesis

Laura L. Grochowski, Huimin Xu, and Robert H. White*

Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308

Received 4 January 2006/ Accepted 7 February 2006

One of the early steps in the biosynthesis of coenzyme F420 in Methanocaldococcus jannaschii requires generation of 2-phospho-L-lactate, which is formed by the phosphorylation of L-lactate. Preliminary studies had shown that L-lactate in M. jannaschii is not derived from pyruvate, and thus an alternate pathway(s) for its formation was examined. Here we report that L-lactate is formed by the NAD+-dependent oxidation of L-lactaldehyde by the MJ1411 gene product. The lactaldehyde, in turn, was found to be generated either by the NAD(P)H reduction of methylglyoxal or by the aldol cleavage of fuculose-1-phosphate by fuculose-1-phosphate aldolase, the MJ1418 gene product.

* Corresponding author. Mailing address: Department of Biochemistry (0308), Virginia Polytechnic Institute and State University, Blacksburg, VA 24061. Phone: (540) 231-6605. Fax: (540) 231-9070. E-mail: rhwhite{at}vt.edu.

Journal of Bacteriology, April 2006, p. 2836-2844, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.2836-2844.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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