This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Penrod, J. T.
Right arrow Articles by Roth, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Penrod, J. T.
Right arrow Articles by Roth, J. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2006, p. 2865-2874, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.2865-2874.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Conserving a Volatile Metabolite: a Role for Carboxysome-Like Organelles in Salmonella enterica

Joseph T. Penrod and John R. Roth*

Microbiology Section, Division of Biological Sciences, University of California, Davis, Davis, California

Received 23 December 2005/ Accepted 30 January 2006

Salmonellae can use ethanolamine (EA) as a sole source of carbon and nitrogen. This ability is encoded by an operon (eut) containing 17 genes, only 6 of which are required under standard conditions (37°C; pH 7.0). Five of the extra genes (eutM, -N, -L, -K, and -G) become necessary under conditions that favor loss of the volatile intermediate, acetaldehyde, which escapes as a gas during growth on EA and is lost at a higher rate from these mutants. The eutM, -N, -L, and -K genes encode homologues of shell proteins of the carboxysome, an organelle shown (in other organisms) to concentrate CO2. We propose that carboxysome-like organelles help bacteria conserve certain volatile metabolites—CO2 or acetaldehyde—perhaps by providing a low-pH compartment. The EutG enzyme converts acetaldehyde to ethanol, which may improve carbon retention by forming acetals; alternatively, EutG may recycle NADH within the carboxysome.

* Corresponding author. Mailing address: Microbiology Section, Division of Biological Sciences, University of California, Davis, Davis, CA. Phone: (530) 752-6679. Fax: (530) 752-7663. E-mail: jrroth{at}ucdavis.edu.

Journal of Bacteriology, April 2006, p. 2865-2874, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.2865-2874.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sagermann, M., Ohtaki, A., Nikolakakis, K. (2009). Crystal structure of the EutL shell protein of the ethanolamine ammonia lyase microcompartment. Proc. Natl. Acad. Sci. USA 106: 8883-8887 [Abstract] [Full Text]  
  • Del Papa, M. F., Perego, M. (2008). Ethanolamine Activates a Sensor Histidine Kinase Regulating Its Utilization in Enterococcus faecalis. J. Bacteriol. 190: 7147-7156 [Abstract] [Full Text]  
  • Sriramulu, D. D., Liang, M., Hernandez-Romero, D., Raux-Deery, E., Lunsdorf, H., Parsons, J. B., Warren, M. J., Prentice, M. B. (2008). Lactobacillus reuteri DSM 20016 Produces Cobalamin-Dependent Diol Dehydratase in Metabolosomes and Metabolizes 1,2-Propanediol by Disproportionation. J. Bacteriol. 190: 4559-4567 [Abstract] [Full Text]  
  • Sampson, E. M., Bobik, T. A. (2008). Microcompartments for B12-Dependent 1,2-Propanediol Degradation Provide Protection from DNA and Cellular Damage by a Reactive Metabolic Intermediate. J. Bacteriol. 190: 2966-2971 [Abstract] [Full Text]  
  • Tanaka, S., Kerfeld, C. A., Sawaya, M. R., Cai, F., Heinhorst, S., Cannon, G. C., Yeates, T. O. (2008). Atomic-Level Models of the Bacterial Carboxysome Shell. Science 319: 1083-1086 [Abstract] [Full Text]  
  • Seedorf, H., Fricke, W. F., Veith, B., Bruggemann, H., Liesegang, H., Strittmatter, A., Miethke, M., Buckel, W., Hinderberger, J., Li, F., Hagemeier, C., Thauer, R. K., Gottschalk, G. (2008). The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features. Proc. Natl. Acad. Sci. USA 105: 2128-2133 [Abstract] [Full Text]  
  • Liu, Y., Leal, N. A., Sampson, E. M., Johnson, C. L. V., Havemann, G. D., Bobik, T. A. (2007). PduL Is an Evolutionarily Distinct Phosphotransacylase Involved in B12-Dependent 1,2-Propanediol Degradation by Salmonella enterica Serovar Typhimurium LT2. J. Bacteriol. 189: 1589-1596 [Abstract] [Full Text]  
  • Heinhorst, S., Williams, E. B., Cai, F., Murin, C. D., Shively, J. M., Cannon, G. C. (2006). Characterization of the Carboxysomal Carbonic Anhydrase CsoSCA from Halothiobacillus neapolitanus. J. Bacteriol. 188: 8087-8094 [Abstract] [Full Text]  
  • Grose, J. H., Joss, L., Velick, S. F., Roth, J. R. (2006). Evidence that feedback inhibition of NAD kinase controls responses to oxidative stress. Proc. Natl. Acad. Sci. USA 103: 7601-7606 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»