This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marchès, O. Articles by Frankel, G. Search for Related Content PubMed PubMed Citation Articles by Marchès, O. Articles by Frankel, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2006, p. 3110-3115, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.3110-3115.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

EspF of Enteropathogenic Escherichia coli Binds Sorting Nexin 9

Division of Cell and Molecular Biology,¹ Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, United Kingdom,² Institute of Child Health, University of Birmingham, Birmingham B4 6NH, United Kingdom,³ Division of Food Sciences, School of Biosciences, University of Nottingham, Loughborough LE 12 5DR, United Kingdom,⁴ Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan,⁵ Department of Medical Biochemistry and Biophysics, Umea University, S-901 87, Umea, Sweden⁶

Received 25 November 2005/ Accepted 6 February 2006

EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal SH3 region. Coimmunoprecipitation and confocal microscopy showed specific EspF-SNX9 interaction and non-mitochondrial protein colocalization in infected epithelial cells.

This article has been cited by other articles:

• Peralta-Ramirez, J., Hernandez, J. M., Manning-Cela, R., Luna-Munoz, J., Garcia-Tovar, C., Nougayrede, J.-P., Oswald, E., Navarro-Garcia, F. (2008). EspF Interacts with Nucleation-Promoting Factors To Recruit Junctional Proteins into Pedestals for Pedestal Maturation and Disruption of Paracellular Permeability. Infect. Immun. 76: 3854-3868 [Abstract] [Full Text]  
• Bujny, M. V., Ewels, P. A., Humphrey, S., Attar, N., Jepson, M. A., Cullen, P. J. (2008). Sorting nexin-1 defines an early phase of Salmonella-containing vacuole-remodeling during Salmonella infection. J. Cell Sci. 121: 2027-2036 [Abstract] [Full Text]  
• Viswanathan, V. K., Weflen, A., Koutsouris, A., Roxas, J. L., Hecht, G. (2008). Enteropathogenic E. coli-induced barrier function alteration is not a consequence of host cell apoptosis. Am. J. Physiol. Gastrointest. Liver Physiol. 294: G1165-G1170 [Abstract] [Full Text]  
• Alto, N. M., Weflen, A. W., Rardin, M. J., Yarar, D., Lazar, C. S., Tonikian, R., Koller, A., Taylor, S. S., Boone, C., Sidhu, S. S., Schmid, S. L., Hecht, G. A., Dixon, J. E. (2007). The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways. J. Cell Biol. 178: 1265-1278 [Abstract] [Full Text]  
• Badour, K., McGavin, M. K. H., Zhang, J., Freeman, S., Vieira, C., Filipp, D., Julius, M., Mills, G. B., Siminovitch, K. A. (2007). Interaction of the Wiskott-Aldrich syndrome protein with sorting nexin 9 is required for CD28 endocytosis and cosignaling in T cells. Proc. Natl. Acad. Sci. USA 104: 1593-1598 [Abstract] [Full Text]