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An Allele of gyrA Prevents Salmonella enterica Serovar Typhimurium from Using Succinate as a Carbon Source

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 13 January 2006/ Accepted 3 February 2006

A mutant gyrA allele resulting in an A271E substitution in the DNA gyrase protein generated a strain unable to grow on the C₄-dicarboxylates succinate, malate, and fumarate. Bacteria harboring gyrA751 displayed decreased negative supercoiling in cells. Expression of the dctA gene, which encodes the C₄-dicarboxylate transporter, was reduced in a gyrA751 mutant, providing the first evidence that dctA expression is supercoiling sensitive and uncovering a simple metabolic screen for lesions in gyrase that reduce negative supercoiling.

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