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Journal of Bacteriology, April 2006, p. 3126-3129, Vol. 188, No. 8
0021-9193/06/$08.00+0 doi:10.1128/JB.188.8.3126-3129.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Department of Bacteriology, University of WisconsinMadison, Madison, Wisconsin 53706
Received 13 January 2006/ Accepted 3 February 2006
A mutant gyrA allele resulting in an A271E substitution in the DNA gyrase protein generated a strain unable to grow on the C4-dicarboxylates succinate, malate, and fumarate. Bacteria harboring gyrA751 displayed decreased negative supercoiling in cells. Expression of the dctA gene, which encodes the C4-dicarboxylate transporter, was reduced in a gyrA751 mutant, providing the first evidence that dctA expression is supercoiling sensitive and uncovering a simple metabolic screen for lesions in gyrase that reduce negative supercoiling.
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