This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wood, L. F.
Right arrow Articles by Ohman, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wood, L. F.
Right arrow Articles by Ohman, D. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2006, p. 3134-3137, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.3134-3137.2006

Independent Regulation of MucD, an HtrA-Like Protease in Pseudomonas aeruginosa, and the Role of Its Proteolytic Motif in Alginate Gene Regulation

Lynn F. Wood and Dennis E. Ohman*

Department of Microbiology and Immunology, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, Virginia 23298-0678, and McGuire Veterans Affairs Medical Center, Richmond, Virginia 23249

Received 19 December 2005/ Accepted 9 February 2006

Expression of mucD, encoding a homologue of the HtrA(DegP) family of endoserine proteases, was investigated in Pseudomonas aeruginosa. Expressed from the algT-mucABCD operon, MucD was detected in mucoid (FRD1) and nonmucoid (PAO1) parental strains and also when polar insertions were placed upstream in algT or mucB. A transcriptional start site for a mucD promoter (PmucD) was mapped within mucC. Expression of single-copy mucD217, encoding MucD altered in the protease motif (S217A), was defective in temperature resistance and alginate gene regulation.

* Corresponding author. Mailing address: Dept. of Microbiology and Immunology, Virginia Commonwealth University, Medical College of Virginia Campus, 1101 E. Marshall St., 5-047 Sanger Hall, P.O. Box 980678, Richmond, VA 23298-0678. Phone: (804) 828-9728. Fax: (804) 828-9946. E-mail: deohman{at}vcu.edu.

Journal of Bacteriology, April 2006, p. 3134-3137, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.3134-3137.2006




This article has been cited by other articles:

  • Tashiro, Y., Sakai, R., Toyofuku, M., Sawada, I., Nakajima-Kambe, T., Uchiyama, H., Nomura, N. (2009). Outer Membrane Machinery and Alginate Synthesis Regulators Control Membrane Vesicle Production in Pseudomonas aeruginosa. J. Bacteriol. 191: 7509-7519 [Abstract] [Full Text]  
  • Ciofu, O., Lee, B., Johannesson, M., Hermansen, N. O., Meyer, P., Hoiby, N., the Scandinavian Cystic Fibrosis Study Consortium, (2008). Investigation of the algT operon sequence in mucoid and non-mucoid Pseudomonas aeruginosa isolates from 115 Scandinavian patients with cystic fibrosis and in 88 in vitro non-mucoid revertants. Microbiology 154: 103-113 [Abstract] [Full Text]  
  • Schenk, A., Berger, M., Keith, L. M., Bender, C. L., Muskhelishvili, G., Ullrich, M. S. (2006). The algT Gene of Pseudomonas syringae pv. glycinea and New Insights into the Transcriptional Organization of the algT-muc Gene Cluster. J. Bacteriol. 188: 8013-8021 [Abstract] [Full Text]  
  • Bragonzi, A., Wiehlmann, L., Klockgether, J., Cramer, N., Worlitzsch, D., Doring, G., Tummler, B. (2006). Sequence diversity of the mucABD locus in Pseudomonas aeruginosa isolates from patients with cystic fibrosis.. Microbiology 152: 3261-3269 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»