This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, C. S.
Right arrow Articles by Gilna, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, C. S.
Right arrow Articles by Gilna, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2006, p. 3382-3390, Vol. 188, No. 9
0021-9193/06/$08.00+0     doi:10.1128/JB.188.9.3382-3390.2006

Pathogenomic Sequence Analysis of Bacillus cereus and Bacillus thuringiensis Isolates Closely Related to Bacillus anthracis{dagger}

Cliff S. Han,1,3,{ddagger} Gary Xie,1,3,{ddagger} Jean F. Challacombe,1,3,{ddagger}* Michael R. Altherr,3 Smriti S. Bhotika,1,3,§ David Bruce,1,3 Connie S. Campbell,1,3 Mary L. Campbell,1,3 Jin Chen,1,3,|| Olga Chertkov,1,3 Cathy Cleland,6 Mira Dimitrijevic,1,3 Norman A. Doggett,3 John J. Fawcett,1,3 Tijana Glavina,2,4 Lynne A. Goodwin,1,3 Karen K. Hill,3 Penny Hitchcock,6,¶ Paul J. Jackson,3,5 Paul Keim,7 Avinash Ramesh Kewalramani,1,3 Jon Longmire,3 Susan Lucas,2,5 Stephanie Malfatti,2,5 Kim McMurry,1,3 Linda J. Meincke,1,3 Monica Misra,1,3 Bernice L. Moseman,1,3 Mark Mundt,8 A. Christine Munk,1,3 Richard T. Okinaka,3 B. Parson-Quintana,1,3 Lee Philip Reilly,1,3 Paul Richardson,2,4 Donna L. Robinson,1,3 Eddy Rubin,2,4 Elizabeth Saunders,1,3 Roxanne Tapia,1,3 Judith G. Tesmer,1,3 Nina Thayer,1,3 Linda S. Thompson,1,3 Hope Tice,2,4 Lawrence O. Ticknor,6 Patti L. Wills,1,3 Thomas S. Brettin,1,3 and Paul Gilna1,3

DOE Joint Genome Institute Los Alamos National Laboratory, Los Alamos, New Mexico 87545,1 DOE Joint Genome Institute Production Genome Facility, Walnut Creek, California 94598,2 Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico 87545,3 Lawrence Berkeley National Laboratory, Berkeley, California 94720,4 Lawrence Livermore National Laboratory, Livermore, California 94550,5 Los Alamos National Laboratory Decision Applications Division, Los Alamos, New Mexico 87545,6 Northern Arizona University Department of Biological Sciences, Flagstaff, Arizona 86011-5640,7 Los Alamos National Laboratory, ESA Division, Los Alamos, New Mexico 875458

Received 22 November 2005/ Accepted 13 February 2006

Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of B. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.

* Corresponding author. Mailing address: Department of Energy Joint Genome Institute, Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545. Phone: (505) 665-1485. Fax: (505) 665-3024. E-mail: jchalla{at}lanl.gov.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} C.S.H., G.X., and J.F.C. contributed equally to this study.

§ Present address: University of Florida, Gainesville, FL 32611.

|| Present address: National Cancer Institute, Rockville, Md.

Present address: The Center for Biosecurity of UPMC, Baltimore, MD 21202.

Journal of Bacteriology, May 2006, p. 3382-3390, Vol. 188, No. 9
0021-9193/06/$08.00+0     doi:10.1128/JB.188.9.3382-3390.2006




This article has been cited by other articles:

  • Ross, C. L., Thomason, K. S., Koehler, T. M. (2009). An Extracytoplasmic Function Sigma Factor Controls {beta}-Lactamase Gene Expression in Bacillus anthracis and Other Bacillus cereus Group Species. J. Bacteriol. 191: 6683-6693 [Abstract] [Full Text]  
  • Mukhopadhyay, S., Akmal, A., Stewart, A. C., Hsia, R.-c., Read, T. D. (2009). Identification of Bacillus anthracis Spore Component Antigens Conserved across Diverse Bacillus cereus sensu lato Strains. Mol. Cell. Proteomics 8: 1174-1191 [Abstract] [Full Text]  
  • Baba, T., Kuwahara-Arai, K., Uchiyama, I., Takeuchi, F., Ito, T., Hiramatsu, K. (2009). Complete Genome Sequence of Macrococcus caseolyticus Strain JSCS5402, Reflecting the Ancestral Genome of the Human-Pathogenic Staphylococci. J. Bacteriol. 191: 1180-1190 [Abstract] [Full Text]  
  • Bouillaut, L., Perchat, S., Arold, S., Zorrilla, S., Slamti, L., Henry, C., Gohar, M., Declerck, N., Lereclus, D. (2008). Molecular basis for group-specific activation of the virulence regulator PlcR by PapR heptapeptides. Nucleic Acids Res 36: 3791-3801 [Abstract] [Full Text]  
  • Dong, S., McPherson, S. A., Tan, L., Chesnokova, O. N., Turnbough, C. L. Jr., Pritchard, D. G. (2008). Anthrose Biosynthetic Operon of Bacillus anthracis. J. Bacteriol. 190: 2350-2359 [Abstract] [Full Text]  
  • Kim, W., Kim, J.-Y., Cho, S.-L., Nam, S.-W., Shin, J.-W., Kim, Y.-S., Shin, H.-S. (2008). Glycosyltransferase - a specific marker for the discrimination of Bacillus anthracis from the Bacillus cereus group. J Med Microbiol 57: 279-286 [Abstract] [Full Text]  
  • Cardazzo, B., Negrisolo, E., Carraro, L., Alberghini, L., Patarnello, T., Giaccone, V. (2008). Multiple-Locus Sequence Typing and Analysis of Toxin Genes in Bacillus cereus Food-Borne Isolates. Appl. Environ. Microbiol. 74: 850-860 [Abstract] [Full Text]  
  • Klevan, A., Tourasse, N. J., Stabell, F. B., Kolsto, A.-B., Okstad, O. A. (2007). Exploring the evolution of the Bacillus cereus group repeat element bcr1 by comparative genome analysis of closely related strains. Microbiology 153: 3894-3908 [Abstract] [Full Text]  
  • Swe, P. M., Heng, N. C. K., Ting, Y.-T., Baird, H. J., Carne, A., Tauch, A., Tagg, J. R., Jack, R. W. (2007). ef1097 and ypkK encode enterococcin V583 and corynicin JK, members of a new family of antimicrobial proteins (bacteriocins) with modular structure from Gram-positive bacteria. Microbiology 153: 3218-3227 [Abstract] [Full Text]  
  • Tinsley, E., Khan, S. A. (2007). A Bacillus anthracis-Based In Vitro System Supports Replication of Plasmid pXO2 as Well as Rolling-Circle-Replicating Plasmids. Appl. Environ. Microbiol. 73: 5005-5010 [Abstract] [Full Text]  
  • Zhong, W., Shou, Y., Yoshida, T. M., Marrone, B. L. (2007). Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by Using Pulsed-Field Gel Electrophoresis. Appl. Environ. Microbiol. 73: 3446-3449 [Abstract] [Full Text]  
  • Challacombe, J. F., Altherr, M. R., Xie, G., Bhotika, S. S., Brown, N., Bruce, D., Campbell, C. S., Campbell, M. L., Chen, J., Chertkov, O., Cleland, C., Dimitrijevic, M., Doggett, N. A., Fawcett, J. J., Glavina, T., Goodwin, L. A., Green, L. D., Han, C. S., Hill, K. K., Hitchcock, P., Jackson, P. J., Keim, P., Kewalramani, A. R., Longmire, J., Lucas, S., Malfatti, S., Martinez, D., McMurry, K., Meincke, L. J., Misra, M., Moseman, B. L., Mundt, M., Munk, A. C., Okinaka, R. T., Parson-Quintana, B., Reilly, L. P., Richardson, P., Robinson, D. L., Saunders, E., Tapia, R., Tesmer, J. G., Thayer, N., Thompson, L. S., Tice, H., Ticknor, L. O., Wills, P. L., Gilna, P., Brettin, T. S. (2007). The Complete Genome Sequence of Bacillus thuringiensis Al Hakam. J. Bacteriol. 189: 3680-3681 [Abstract] [Full Text]  
  • Zigha, A., Rosenfeld, E., Schmitt, P., Duport, C. (2007). The Redox Regulator Fnr Is Required for Fermentative Growth and Enterotoxin Synthesis in Bacillus cereus F4430/73. J. Bacteriol. 189: 2813-2824 [Abstract] [Full Text]  
  • Wijman, J. G. E., de Leeuw, P. P. L. A., Moezelaar, R., Zwietering, M. H., Abee, T. (2007). Air-Liquid Interface Biofilms of Bacillus cereus: Formation, Sporulation, and Dispersion. Appl. Environ. Microbiol. 73: 1481-1488 [Abstract] [Full Text]  
  • Rasko, D. A., Rosovitz, M. J., Okstad, O. A., Fouts, D. E., Jiang, L., Cer, R. Z., Kolsto, A.-B., Gill, S. R., Ravel, J. (2007). Complete Sequence Analysis of Novel Plasmids from Emetic and Periodontal Bacillus cereus Isolates Reveals a Common Evolutionary History among the B. cereus-Group Plasmids, Including Bacillus anthracis pXO1. J. Bacteriol. 189: 52-64 [Abstract] [Full Text]  
  • de Been, M., Francke, C., Moezelaar, R., Abee, T., Siezen, R. J. (2006). Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis.. Microbiology 152: 3035-3048 [Abstract] [Full Text]  
  • Choudhury, B., Leoff, C., Saile, E., Wilkins, P., Quinn, C. P., Kannenberg, E. L., Carlson, R. W. (2006). The Structure of the Major Cell Wall Polysaccharide of Bacillus anthracis Is Species-specific. J. Biol. Chem. 281: 27932-27941 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»