This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Magnet, S.
Right arrow Articles by Gutmann, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Magnet, S.
Right arrow Articles by Gutmann, L.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2007, p. 3927-3931, Vol. 189, No. 10
0021-9193/07/$08.00+0     doi:10.1128/JB.00084-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of the L,D-Transpeptidases Responsible for Attachment of the Braun Lipoprotein to Escherichia coli Peptidoglycan{triangledown}

Sophie Magnet,1,2,3* Samuel Bellais,1,2,3,{dagger} Lionel Dubost,5 Martine Fourgeaud,1,2,3 Jean-Luc Mainardi,1,2,3,4 Sébastien Petit-Frère,1,2,3 Arul Marie,5 Dominique Mengin-Lecreulx,6 Michel Arthur,1,2,3 and Laurent Gutmann1,2,3,4

INSERM, U655-LRMA, Paris, F-75006 France,1 Université Pierre et Marie Curie-Paris 6, Centre de Recherches Biomédicales des Cordeliers, Paris, F-75006 France,2 Université Paris-Descartes, Faculté de Médecine René Descartes, Centre de Recherches Biomédicales des Cordeliers, Paris, F-75006 France,3 AP-HP, Hôpital Européen Georges Pompidou, Paris, F-75015 France,4 Muséum National d'Histoire Naturelle, Plateforme de Spectrométrie de Masse et de Protéomique, Département Recherche Développement et Diversité Moléculaire, Paris, F-75005 France,5 Laboratoire des Enveloppes Bactériennes et Antibiotiques, UMR 8619 CNRS, Université Paris-Sud, 91405 Orsay, France6

Received 16 January 2007/ Accepted 9 March 2007

The L,D-transpeptidase Ldtfm catalyzes peptidoglycan cross-linking in ß-lactam-resistant mutant strains of Enterococcus faecium. Here, we show that in Escherichia coli Ldtfm homologues are responsible for the attachment of the Braun lipoprotein to murein, indicating that evolutionarily related domains have been tailored to use muropeptides or proteins as acyl acceptors in the L,D-transpeptidation reaction.

* Corresponding author. Mailing address: INSERM U655-LRMA, Université Pierre et Marie Curie Centre de Recherches Biomédicales des Cordeliers, 15 rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France. Phone: 33 1 42 34 68 62. Fax: 33 1 43 25 68 12. E-mail: sophie.magnet{at}bhdc.jussieu.fr

{triangledown} Published ahead of print on 16 March 2007.

{dagger} Present address: INSERM U781, Hôpital Necker Enfants Malades, 75015, Paris, France.

Journal of Bacteriology, May 2007, p. 3927-3931, Vol. 189, No. 10
0021-9193/07/$08.00+0     doi:10.1128/JB.00084-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Depuydt, M., Leonard, S. E., Vertommen, D., Denoncin, K., Morsomme, P., Wahni, K., Messens, J., Carroll, K. S., Collet, J.-F. (2009). A Periplasmic Reducing System Protects Single Cysteine Residues from Oxidation. Science 326: 1109-1111 [Abstract] [Full Text]  
  • Magnet, S., Dubost, L., Marie, A., Arthur, M., Gutmann, L. (2008). Identification of the L,D-Transpeptidases for Peptidoglycan Cross-Linking in Escherichia coli. J. Bacteriol. 190: 4782-4785 [Abstract] [Full Text]  
  • Lavollay, M., Arthur, M., Fourgeaud, M., Dubost, L., Marie, A., Veziris, N., Blanot, D., Gutmann, L., Mainardi, J.-L. (2008). The Peptidoglycan of Stationary-Phase Mycobacterium tuberculosis Predominantly Contains Cross-Links Generated by L,D-Transpeptidation. J. Bacteriol. 190: 4360-4366 [Abstract] [Full Text]  
  • Mainardi, J.-L., Hugonnet, J.-E., Rusconi, F., Fourgeaud, M., Dubost, L., Moumi, A. N., Delfosse, V., Mayer, C., Gutmann, L., Rice, L. B., Arthur, M. (2007). Unexpected Inhibition of Peptidoglycan LD-Transpeptidase from Enterococcus faecium by the beta-Lactam Imipenem. J. Biol. Chem. 282: 30414-30422 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»