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Journal of Bacteriology, June 2007, p. 4135-4140, Vol. 189, No. 11
0021-9193/07/$08.00+0 doi:10.1128/JB.00078-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
-Glucose Substitution of Poly(Glycerophosphate) Backbones
Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland,1 Department of Biochemical Pharmacology, University of Konstanz, D-78457 Konstanz, Germany,2 Institute for Organic Chemistry, Philipps-Universität Marburg, D-35043 Marburg, Germany,3 Department Biologie I, Bereich Mikrobiologie der Universität München, D-80638 Munich, Germany,4 Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo, Finland,5 European Centre for the Validation of Alternative Methods, IHCP, JRC, 21020 Ispra, Italy,6 Department of Food Technology, University of Helsinki, P.O. Box 66, FIN-00014 Helsinki, Finland7
Received 16 January 2007/ Accepted 28 March 2007
Lipoteichoic acids (LTAs) have been shown to act as bacterial counterparts to the receptor binding proteins of LL-H, LL-H host range mutant LL-H-a21, and JCL1032. Here we have used LTAs purified by hydrophobic interaction chromatography from different phage-resistant and -sensitive strains of Lactobacillus delbrueckii subsp. lactis. Nuclear magnetic resonance analyses revealed variation in the degree of
-glucosyl and D-alanyl substitution of the 1,3-linked poly(glycerophosphate) LTAs between the phage-sensitive and phage-resistant strains. Inactivation of phages was less effective if there was a high level of D-alanine residues in the LTA backbones. Prior incubation of the LTAs with
-glucose-specific lectin inhibited the LL-H phage inactivation. The overall level of decoration or the specific spatial combination of
-glucosyl-substituted, D-alanyl-substituted, and nonsubstituted glycerol residues may also affect phage adsorption.
Published ahead of print on 6 April 2007.
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