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Journal of Bacteriology, June 2007, p. 4135-4140, Vol. 189, No. 11
0021-9193/07/$08.00+0     doi:10.1128/JB.00078-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Interaction between Lipoteichoic Acids and Lactobacillus delbrueckii Phages Depends on D-Alanyl and -Glucose Substitution of Poly(Glycerophosphate) Backbones

Liisa Räisänen,^1* Christian Draing,² Markus Pfitzenmaier,³ Karin Schubert,⁴ Tiina Jaakonsaari,⁵ Sonja von Aulock,² Thomas Hartung,^2,6 and Tapani Alatossava⁷

Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland,¹ Department of Biochemical Pharmacology, University of Konstanz, D-78457 Konstanz, Germany,² Institute for Organic Chemistry, Philipps-Universität Marburg, D-35043 Marburg, Germany,³ Department Biologie I, Bereich Mikrobiologie der Universität München, D-80638 Munich, Germany,⁴ Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo, Finland,⁵ European Centre for the Validation of Alternative Methods, IHCP, JRC, 21020 Ispra, Italy,⁶ Department of Food Technology, University of Helsinki, P.O. Box 66, FIN-00014 Helsinki, Finland⁷

Received 16 January 2007/ Accepted 28 March 2007

Lipoteichoic acids (LTAs) have been shown to act as bacterial counterparts to the receptor binding proteins of LL-H, LL-H host range mutant LL-H-a21, and JCL1032. Here we have used LTAs purified by hydrophobic interaction chromatography from different phage-resistant and -sensitive strains of Lactobacillus delbrueckii subsp. lactis. Nuclear magnetic resonance analyses revealed variation in the degree of -glucosyl and D-alanyl substitution of the 1,3-linked poly(glycerophosphate) LTAs between the phage-sensitive and phage-resistant strains. Inactivation of phages was less effective if there was a high level of D-alanine residues in the LTA backbones. Prior incubation of the LTAs with -glucose-specific lectin inhibited the LL-H phage inactivation. The overall level of decoration or the specific spatial combination of -glucosyl-substituted, D-alanyl-substituted, and nonsubstituted glycerol residues may also affect phage adsorption.

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* Corresponding author. Mailing address: Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland. Phone: 358-8-553 1786. Fax: 358-8-553 1061. E-mail: liisa.raisanen{at}oulu.fi

Published ahead of print on 6 April 2007.

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Journal of Bacteriology, June 2007, p. 4135-4140, Vol. 189, No. 11
0021-9193/07/$08.00+0     doi:10.1128/JB.00078-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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