This Article Full Text Full Text (PDF) Other Versions of this Article: JB.00078-07v1 189/11/4135    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Räisänen, L. Articles by Alatossava, T. Search for Related Content PubMed PubMed Citation Articles by Räisänen, L. Articles by Alatossava, T.

Molecular Interaction between Lipoteichoic Acids and Lactobacillus delbrueckii Phages Depends on D-Alanyl and -Glucose Substitution of Poly(Glycerophosphate) Backbones

Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland,¹ Department of Biochemical Pharmacology, University of Konstanz, D-78457 Konstanz, Germany,² Institute for Organic Chemistry, Philipps-Universität Marburg, D-35043 Marburg, Germany,³ Department Biologie I, Bereich Mikrobiologie der Universität München, D-80638 Munich, Germany,⁴ Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo, Finland,⁵ European Centre for the Validation of Alternative Methods, IHCP, JRC, 21020 Ispra, Italy,⁶ Department of Food Technology, University of Helsinki, P.O. Box 66, FIN-00014 Helsinki, Finland⁷

Received 16 January 2007/ Accepted 28 March 2007

Lipoteichoic acids (LTAs) have been shown to act as bacterial counterparts to the receptor binding proteins of LL-H, LL-H host range mutant LL-H-a21, and JCL1032. Here we have used LTAs purified by hydrophobic interaction chromatography from different phage-resistant and -sensitive strains of Lactobacillus delbrueckii subsp. lactis. Nuclear magnetic resonance analyses revealed variation in the degree of -glucosyl and D-alanyl substitution of the 1,3-linked poly(glycerophosphate) LTAs between the phage-sensitive and phage-resistant strains. Inactivation of phages was less effective if there was a high level of D-alanine residues in the LTA backbones. Prior incubation of the LTAs with -glucose-specific lectin inhibited the LL-H phage inactivation. The overall level of decoration or the specific spatial combination of -glucosyl-substituted, D-alanyl-substituted, and nonsubstituted glycerol residues may also affect phage adsorption.

Published ahead of print on 6 April 2007.

This article has been cited by other articles:

• Baptista, C., Santos, M. A., Sao-Jose, C. (2008). Phage SPP1 Reversible Adsorption to Bacillus subtilis Cell Wall Teichoic Acids Accelerates Virus Recognition of Membrane Receptor YueB. J. Bacteriol. 190: 4989-4996 [Abstract] [Full Text]  
• Seo, H. S., Cartee, R. T., Pritchard, D. G., Nahm, M. H. (2008). A New Model of Pneumococcal Lipoteichoic Acid Structure Resolves Biochemical, Biosynthetic, and Serologic Inconsistencies of the Current Model. J. Bacteriol. 190: 2379-2387 [Abstract] [Full Text]